In this study, the anti-oxidant and anti-bacterial activity of marine brown algae Turbinaria ornata (T. ornate) extract was purified by soxhlet apparatus method. The invitro studies of total anti-oxidant activity and DPPH hydroxyl scavenging activity assays were suggested that the T. ornata extract has more anti-oxidant activity. Agar well diffusion and minimum inhibition concentration results were exhibited excellent inhibition effect of T. ornata extract against P. aeruginosa at 300 µg/mL concentration. The time-dependent bacterial inhibition assay was also confirmed the 300 µg/mL concentration at 24 h. Further, the increased death cells and extracellular shape alteration results were observed by confocal laser scanning electron microscope and scanning electron microscope. Therefore, our result was suggested that the T. ornata extract as a promising anti-bacterial agent for multi-drug resistant P. aeruginosa.

在这项研究中，抗氧化剂和海洋褐藻抗细菌活性Turbinaria姬蛙（T.华丽的）提取物通过索格利特装置的方法进行纯化。在体外总抗氧化活性和DPPH自由基的清除羟基活性测定的研究，建议T.姬蛙提取物具有更多的抗氧化活性。琼脂的良好扩散和最小抑菌浓度结果显示了紫草提取物对铜绿假单胞菌的优异抑制作用浓度为300 µg / mL。还证实了时间依赖性细菌抑制测定法在24 h浓度为300 µg / mL。此外，通过共聚焦激光扫描电子显微镜和扫描电子显微镜观察到死亡细胞增加和细胞外形状改变的结果。因此，我们的结果表明，紫草提取物作为一种对多药耐药的铜绿假单胞菌具有前景的抗菌剂。