Abstract Aristotelia serrata, wineberry, or makomako, is a small deciduous fast-growing shrub of New Zealand that produces a berry. In this study leaf extracts were produced using ultrasound technology. Alzheimer’s Disease is a progressive mental deterioration related with ageing and senility, in which approved drugs for treatment inhibit butyrylcholinesterase (BChE), an enzyme present in the human brain. The ultrasound assisted extraction of natural anti-butyrylcholinesterase and antioxidants from this plant, and the yield of extraction were optimized using response surface methodology. The effect of acoustic power density (0.13-0.65 W/mL), time (2-20 min), and ethanol concentration (20-80%) on BChE inhibition, antioxidant activity, and extraction yield was determined, the optimum extraction conditions being 0.53 W/mL, 17.12 min and 74% ethanol. The optimized extracts were produced to experimentally validate the predicted responses. The IC50 concentrations were experimentally determined (26.2 μg/mL for BChE, 5.5 μg/mL for radical scavenging activity). A. serrata leaf extracts have potential to be used as complements to medicines traditionally used for memory dysfunction.

中文翻译：

---

超声提取对锯齿亚里士多叶提取物丁酰胆碱酯酶抑制作用及药用特性的改善

摘要 Aristotelia serrata、wineberry 或 makomako 是新西兰的一种小型落叶速生灌木，可生产浆果。在这项研究中，叶提取物是使用超声波技术生产的。阿尔茨海默病是一种与衰老和衰老相关的进行性精神恶化，其中批准的治疗药物会抑制丁酰胆碱酯酶 (BChE)，这是一种存在于人脑中的酶。超声辅助从该植物中提取天然抗丁酰胆​​碱酯酶和抗氧化剂，并使用响应面法优化提取率。确定了声功率密度 (0.13-0.65 W/mL)、时间 (2-20 min) 和乙醇浓度 (20-80%) 对 BChE 抑制、抗氧化活性和提取率的影响，最佳提取条件为0.53 W/mL，17.12 分钟和 74% 乙醇。产生优化的提取物以通过实验验证预测的响应。通过实验确定 IC50 浓度（BChE 为 26.2 μg/mL，自由基清除活性为 5.5 μg/mL）。A. serrata 叶提取物有可能用作传统上用于记忆功能障碍的药物的补充剂。