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Fabrication and Investigation of the Suitability of Chitosan-Silver Composite Scaffolds for Bone Tissue Engineering Applications
Process Biochemistry ( IF 4.4 ) Pub Date : 2021-01-01 , DOI: 10.1016/j.procbio.2020.10.008
Baskaran Vaidhyanathan , Preethi Vincent , Sajini Vadivel , Ponmurugan Karuppiah , Naif Abdullah AL-Dhabi , Deepa Rani Sadhasivam , Selvaraj Vimalraj , Sekaran Saravanan

Abstract Scaffolds fabricated with only chitosan (CS) as base polymer is limited by its fast degradation rate, reduced mechanical strength and diminished bioactivity. To achieve optimal properties suitable for bone tissue engineering, CS based scaffolds are developed by addition of copolymers, nanoparticles, and functional group modifications. In our study, biodegradable composite scaffolds were fabricated by utilizing CS and silver nanoparticles (AgNPs) were in-situ synthesised on CS matrix with no external reducing agent. The scaffold was investigated for its suitability to be employed in bone tissue engineering (BTE) applications. The scaffolds were found to be highly porous and possess the ability of hydration, adsorb proteins and biomineralization. Chelation with silver ions greatly improved the properties of CS scaffolds by controlling swelling and degradation rate. In addition this, the scaffolds were found to exert broad spectrum antibacterial activity. Furthermore, the scaffolds are highly biocompatible and supported the growth of osteoblast. It also promoted osteogenic differentiation by upregulating Runt-related transcription factor 2 (Runx2), Type-1 collagen (Col-I), Alkaline phosphatase (ALP) activity and secreted Osteocalcin (OC) levels. Among the synthesized scaffolds, CS-Ag 1.5 M scaffold was found to be superior and suitable for bone tissue engineering applications.

中文翻译:

用于骨组织工程应用的壳聚糖-银复合支架的制备和适用性研究

摘要 仅以壳聚糖 (CS) 为基础聚合物制备的支架因其降解速度快、机械强度降低和生物活性降低而受到限制。为了获得适合骨组织工程的最佳性能,通过添加共聚物、纳米颗粒和官能团修饰来开发基于 CS 的支架。在我们的研究中,利用 CS 制造了可生物降解的复合支架,并在没有外部还原剂的情况下在 CS 基质上原位合成银纳米粒子 (AgNPs)。研究了该支架在骨组织工程 (BTE) 应用中的适用性。发现支架具有高度多孔性,并具有水合作用、吸附蛋白质和生物矿化的能力。与银离子螯合通过控制溶胀和降解速率极大地改善了 CS 支架的性能。此外,发现支架具有广谱抗菌活性。此外,支架具有高度的生物相容性并支持成骨细胞的生长。它还通过上调 Runt 相关转录因子 2 (Runx2)、1 型胶原蛋白 (Col-I)、碱性磷酸酶 (ALP) 活性和分泌的骨钙素 (OC) 水平来促进成骨分化。在合成的支架中,发现 CS-Ag 1.5 M 支架性能优越,适用于骨组织工程应用。它还通过上调 Runt 相关转录因子 2 (Runx2)、1 型胶原蛋白 (Col-I)、碱性磷酸酶 (ALP) 活性和分泌的骨钙素 (OC) 水平来促进成骨分化。在合成的支架中,发现 CS-Ag 1.5 M 支架性能优越,适用于骨组织工程应用。它还通过上调 Runt 相关转录因子 2 (Runx2)、1 型胶原蛋白 (Col-I)、碱性磷酸酶 (ALP) 活性和分泌的骨钙素 (OC) 水平来促进成骨分化。在合成的支架中,发现 CS-Ag 1.5 M 支架性能优越,适用于骨组织工程应用。
更新日期:2021-01-01
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