The screening of 862 T-DNA lines was carried out to approach the genetic dissection of indirect adventitious organogenesis in tomato. Several mutants defective in different phases of adventitious organogenesis, namely callus growth (tdc-1), bud differentiation (tdb-1, -2, -3) and shoot-bud development (tds-1) were identified and characterized. The alteration of the TDC-1 gene blocked callus proliferation depending on the composition of growth regulators in the culture medium. Calli from tds-1 explants differentiated buds but did not develop normal shoots. Histological analysis showed that their abnormal development is due to failure in the organization of normal adventitious shoot meristems. Interestingly, tdc-1 and tds-1 mutant plants were indistinguishable from WT ones, indicating that the respective altered genes play specific roles in cell proliferation from explant cut zones (TDC-1 gene) or in the organization of adventitious shoot meristems (TDS-1 gene). Unlike the previous, plants of the three mutants defective in the differentiation of adventitious shoot-buds (tdb-1, -2, -3) showed multiple changes in vegetative and reproductive traits. Cosegregation analyses revealed the existence of an association between the phenotype of the tdb-3 mutant and a T-DNA insert, which led to the discovery that the SlMAPKKK17 gene is involved in the shoot-bud differentiation process.

中文翻译：

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番茄外植体间接不定器官发生过程的遗传剖析

进行了 862 个 T-DNA 系的筛选，以接近番茄间接不定器官发生的遗传解剖。鉴定并表征了在不定器官发生的不同阶段存在缺陷的几种突变体，即愈伤组织生长 (tdc-1)、芽分化 (tdb-1、-2、-3) 和芽芽发育 (tds-1)。根据培养基中生长调节剂的组成，TDC-1 基因的改变会阻止愈伤组织增殖。来自 tds-1 外植体的愈伤组织分化出芽，但没有发育出正常的芽。组织学分析表明，它们的异常发育是由于正常不定枝分生组织的组织失败。有趣的是，tdc-1 和 tds-1 突变植物与 WT 植物无法区分，表明各自改变的基因在外植体切割区（TDC-1 基因）的细胞增殖或不定枝分生组织（TDS-1 基因）的组织中发挥特定作用。与以前不同的是，不定芽芽分化缺陷的三个突变体（tdb-1、-2、-3）的植物在营养和繁殖性状上表现出多重变化。共分离分析揭示了 tdb-3 突变体的表型与 T-DNA 插入物之间存在关联，这导致发现 SlMAPKKK17 基因参与芽芽分化过程。-3) 在营养和繁殖性状上表现出多重变化。共分离分析揭示了 tdb-3 突变体的表型与 T-DNA 插入物之间存在关联，这导致发现 SlMAPKKK17 基因参与芽芽分化过程。-3) 在营养和繁殖性状上表现出多重变化。共分离分析揭示了 tdb-3 突变体的表型与 T-DNA 插入物之间存在关联，这导致发现 SlMAPKKK17 基因参与芽芽分化过程。