This study systematically investigates the role of graphene oxide (GO) and reduced GO (rGO)/silk-based composite micro/nano-fibrous scaffolds in regulating neuronal cell behavior in vitro, given the limited comparative studies on the effects of graphene family materials on nerve regeneration. Fibrous scaffolds can mimic the architecture of the native extracellular matrix and are potential candidates for tissue engineering peripheral nerves. Silk/GO micro/nano-fibrous scaffolds were electrospun with GO loadings 1 to 10 wt.%, and optionally post-reduced in situ to explore a family of electrically conductive non-woven silk/rGO scaffolds. Conductivities up to 4 × 10⁻⁵ S cm⁻¹ were recorded in the dry state, which increased up to 3 × 10⁻⁴ S cm⁻¹ after hydration. Neuronoma NG108-15 cells adhered and were viable on all substrates. Enhanced metabolic activity and proliferation were observed on the GO-containing scaffolds, and these cell responses were further promoted for electroactive silk/rGO. Neurite extensions up to 100 μm were achieved by day 5, with maximum outgrowth up to ~250 μm on some of the conductive substrates. These electroactive composite fibrous scaffolds exhibit potential to enhance the neuronal cell response and could be versatile supportive substrates for neural tissue engineering applications.

本研究系统地研究了氧化石墨烯（GO）和还原的GO（rGO）/基于丝的复合微/纳米纤维支架在体外调节神经元细胞行为中的作用，但有关石墨烯家族材料对血管生成的影响的比较研究有限。神经再生。纤维支架可以模拟天然细胞外基质的结构，并且是组织工程性周围神经的潜在候选者。将丝/ GO微/纳米纤维支架电纺丝，GO负载为1至10 wt。％，并可选地在原位进行后还原，以探索一类导电性非织造丝/ rGO支架。在干燥状态下记录的电导率高达4×10 ^-5  S cm ^-1，增加到3×10 ^-4  S cm ^-1水合后。神经瘤NG108-15细胞粘附并在所有底物上均存活。在含有GO的支架上观察到增强的代谢活性和增殖，并且对于电活性丝/ rGO，这些细胞反应被进一步促进。到第5天，神经突延伸达到100μm，在某些导电基底上的最大向外生长达到〜250μm。这些电活性复合纤维支架显示出增强神经元细胞反应的潜力，并且可以作为神经组织工程应用的多功能支持基质。