Tryptamines are hallucinogenic substances many of which have appeared recently as novel psychoactive substances (NPS). Herein, we describe the establishment of a rapid UHPLC-MS/MS quantitative method for the targeted screening of 16 tryptamines of abuse in hair. Twenty milligram pieces of hair were pulverized below 4 °C in 0.5 mL of deionized water containing 0.1% formic acid and an internal standard (2 ng/mL psilocin-d₁₀ and psilocybin-d₄). After subsequent centrifugation, 5 μL of the supernatant was injected into a LC–MS/MS system fitted with a Waters Acquity UPLC HSS T₃ column (100 mm × 2.1 mm, 1.8 mm). The column was gradient eluted at 0.3 mL/min with mobile phases composed of 20 mmol/L ammonium acetate, 5% acetonitrile, and 0.1% formic acid in water (solvent A) and acetonitrile (solvent B). Limits of detection ranged between 0.1 and 20 pg/mg, with limits of quantitation ranging from 3 to 50 pg/mg. The calibration curves for all analytes were linear (r >0.992). Accuracies varied between 91% and 114%, with intraday precision RSDs < 14% and interday precision RSDs of between 1.3% and 14%. The recoveries of all tryptamines were in the 85–115% range, with the matrix effect ranging from 95% to 112%. The validated method was successfully used to analyse 191 hair samples from suspected tryptamine users, 77 of which were 5-MeO-DiPT-positive, while the 16 tryptamines and their metabolites were not detected in the remaining 114 hair samples. 5-MeO-DiPT and its 5-MeO-NiPT, 5-OH-DiPT, and 4-OH-DiPT metabolites were concurrently detected in 34 hair samples. 5-MeO-DiPT, as the parent drug, was the parent substance found in the hair samples.

色胺是一种致幻物质，其中许多物质最近已作为新型精神活性物质（NPS）出现。在这里，我们描述了一种快速UHPLC-MS / MS定量方法的建立，用于针对性筛选头发中16种色胺的滥用。在低于4°C的温度下，将20毫克的头发在0.5 mL的含0.1％甲酸和内标物（2 ng / mL psilocin-d ₁₀和psilocybin-d ₄）的去离子水中粉碎。在随后的离心之后，将5μL上清液注入装有Waters Acquity UPLC HSS T ₃的LC–MS / MS系统中色谱柱（100毫米×2.1毫米，1.8毫米）。用0.3 mL / min的流速将色谱柱梯度洗脱，流动相由20 mmol / L乙酸铵，5％乙腈和0.1％甲酸的水溶液（溶剂A）和乙腈（溶剂B）组成。检测限为0.1至20 pg / mg，定量限为3至50 pg / mg。所有分析物的校准曲线均为线性（r> 0.992）。精度在91％和114％之间变化，日内精确RSD <14％，日间精确RSD在1.3％和14％之间。所有色胺的回收率在85–115％范围内，基质效应范围从95％至112％。经过验证的方法已成功用于分析191名疑似色胺使用者的头发样本，其中77名是5-MeO-DiPT阳性，而其余114个头发样本中未检测到16种色胺及其代谢产物。在34个头发样本中同时检测到5-MeO-DiPT及其5-MeO-NiPT，5-OH-DiPT和4-OH-DiPT代谢产物。5-MeO-DiPT作为母体药物，是在头发样本中发现的母体物质。