Successful treatment of Acinetobacter baumannii infections require early and appropriate antimicrobial therapy. One of the first steps in this process is understanding which β-lactamase (bla) alleles are present and in what combinations. Thus, we performed WGS on 98 carbapenem-resistant A. baumannii (CR Ab). In most isolates, an acquired bla_OXA carbapenemase was found in addition to the intrinsic bla_OXA allele. The most commonly found allele was bla_OXA-23 (n=78/98). In some isolates, bla_OXA-23 was found in addition to other carbapenemase alleles: bla_OXA-82 (n=12/78), bla_OXA-72 (n=2/78) and bla_OXA-24/40 (n=1/78). Surprisingly, 20% of isolates carried carbapenemases not routinely assayed for by rapid molecular diagnostic platforms, i.e., bla_OXA-82 and bla_OXA-172; all had ISAba1 elements. In 8 CR Ab, bla_OXA-82 or bla_OXA-172 was the only carbapenemase. Both bla_OXA-24/40 and its variant bla_OXA-72 were each found in 6/98 isolates. The most prevalent ADC variants were bla_ADC-30 (21%), bla_ADC-162 (21%), and bla_ADC-212 (26%). Complete combinations are reported.

鲍曼不动杆菌感染的成功治疗需要早期和适当的抗菌治疗。该过程的第一步是了解存在哪些 β-内酰胺酶 (bla) 等位基因以及存在哪些组合。因此，我们对 98 种耐碳青霉烯类鲍曼不动杆菌 (CR Ab) 进行了 WGS。在大多数分离株中， 除了固有的 bla _OXA 等位基因外，还发现了获得性的 bla _OXA碳青霉烯酶。最常见的等位基因是bla _OXA-23  (n=78/98)。在一些分离株中， 除了其他碳青霉烯酶等位基因外，还发现了bla _OXA-23：bla _OXA-82  (n=12/78)、bla _OXA-72  (n=2/78) 和 bla _OXA-24/40 (n=1/78)。令人惊讶的是，20% 的分离株携带的碳青霉烯酶未通过快速分子诊断平台进行常规检测，即 bla _OXA-82 和 bla _OXA-172；都含有 ISAba1 元素。在 8 CR Ab 中，bla _OXA-82 或 bla _OXA-172 是唯一的碳青霉烯酶。bla _OXA-24/40 及其变体 bla _OXA-72 均在 6/98 株中发现。最流行的 ADC 变体是 bla _ADC-30  (21%)、bla _ADC-162  (21%) 和 bla _ADC-212  (26%)。报告了完整的组合。