ASC is a component of the inflammasome playing crucial roles in the inflammatory response. In mammals, ASC induces pyroptosis and inflammatory cytokine production. In this study, three asc genes (asc1, asc2, and asc3) from the Japanese medaka (Oryzias latipes) were identified and characterized. These asc genes were tandem replicates on chromosome 16, and their exon-intron structures differed between them. All three ASCs conserved the pyrin and caspase-recruitment domains, which are important for inflammasome formation. In phylogenetic analysis, all ASCs clustered with those of other teleosts. The asc1 expression levels were significantly higher in several organs than those of asc2 and asc3, suggesting that asc1 may act as a dominant asc in the Japanese medaka. Expression of the three asc genes showed different patterns during Aeromonas hydrophila and Edwardsiella piscicida infections. Furthermore, their expression was adequately down-regulated in the medaka fin-derived cells stimulated with ATP for 12 h, while asc2 expression was statistically up-regulated after nigericin stimulation for 24 h. Moreover, the expression of asc2 and asc3 was significantly higher in the skin of ASC-1-knockout medaka than in that of the wild type medaka during A. hydrophila infection.

ASC 是炎症小体的一个组成部分，在炎症反应中起关键作用。在哺乳动物中，ASC 诱导细胞焦亡和炎性细胞因子的产生。在这项研究中，鉴定和表征了来自日本青鳉 ( Oryzias latipes ) 的三个asc基因（asc1、asc2和asc3）。这些asc 基因是 16 号染色体上的串联复制，它们的外显子-内含子结构不同。所有三个 ASC 都保留了 pyrin 和 caspase 募集结构域，这对于炎性体的形成很重要。在系统发育分析，所有ASC Ş集群与其他硬骨鱼的。该ASC1几个器官中的表达水平显着高于asc2和asc3，这表明asc1可能在日本青鳉中作为主要的asc。三个asc基因的表达在嗜水气单胞菌和爱德华氏菌感染期间表现出不同的模式。此外，它们的表达在用 ATP 刺激 12 小时的青鳉衍生细胞中充分下调，而在尼日利亚菌素刺激 24 小时后，asc2 的表达在统计学上上调。此外，asc2和asc3的表达式ASC-1 基因敲除青鳉的皮肤在A期间显着高于野生型青鳉。嗜水菌感染。