Hydrolysis is considered the limiting stage of the anaerobic digestion of microalgae due to the recalcitrance of this type of biomass. Hydrolysis of microalgae with ruminal fluid, a microbial culture with high hydrolytic capacities, is a promising pretreatment method. In the present work, the long-term hydrolysis process of a native consortium of microalgae (98% Scenedesmus) was studied in a sequential batch reactor inoculated with ruminal fluid. The influence of the hydraulic retention time (HRT) was evaluated in such a way to obtain a hydrolysate rich either in sugars or volatile fatty acids (VFA) and then to produce, in a subsequent stage, hydrogen or methane. Results evidenced that it is possible to maintain the hydrolytic activity for long periods when ruminal fluid is used as the inoculum. Using an HRT of 2 days a hydrolyzate rich in sugars was obtained. The fermentation of this hydrolysate produced 1.7 times more hydrogen than the amount produced with raw microalgae. When the HRT was adjusted to 8 days, the hydrolysis efficiencies increased to 60% and a hydrolysate rich in VFA was obtained. The methanization of this effluent yielded about 81% of the theoretical value. The most abundant bacteria present in the culture were Pseudomonas, Bacteroides, Clostridium, Acinetobacter and Proteocatella. The gaseous biofuel production was improved by hydrolyzing the microalgal biomass with ruminal fluid (up to 2.6 times more than the raw biomass), and it was possible to recover up to 16.8 kJ per gram of volatile solids.

由于这种生物质的顽固性，水解被认为是微藻厌氧消化的限制阶段。瘤胃液水解微藻是一种很有前途的预处理方法，瘤胃液是一种具有高水解能力的微生物培养物。在目前的工作中，一个天然微藻财团的长期水解过程（98％Scenedesmus在接种瘤胃液的连续间歇式反应器中进行了研究。以这样的方式评估水力停留时间（HRT）的影响，以获得富含糖或挥发性脂肪酸（VFA）的水解产物，然后在后续阶段中生产氢气或甲烷。结果证明，当使用瘤胃液作为接种物时，可以长期维持水解活性。使用2天的HRT，获得了富含糖的水解产物。这种水解产物的发酵产生的氢比未加工微藻产生的氢多1.7倍。将HRT调整为8天时，水解效率提高到60％，并获得了富含VFA的水解产物。该流出物的甲烷化产生理论值的约81％。假单胞菌，拟杆菌，梭菌，不动杆菌和变形杆菌。通过使用瘤胃液水解微藻生物质来提高气态生物燃料的产量（比原始生物质高2.6倍），每克挥发性固体最多可回收16.8 kJ。