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Genetic Diversity of Brinjal Fruit and Shoot Borer (BSFB) Population of Odisha, India
Iranian Journal of Science and Technology, Transactions A: Science ( IF 1.7 ) Pub Date : 2020-10-17 , DOI: 10.1007/s40995-020-00997-y
Dattatreya Kar , Ananya Kuanar , Asit Ray , Mahendra Gaur , Bibhudutta Pattanaik , Budhadev Mishra

In Southern and Southern East Asia, the eggplant fruit and shoot borer (BSFB) is the most harmful pest for eggplant. The pest is known to cause about 16 and 70% damage to shoots and fruits, respectively. It is of practical importance to precisely trace the genetic variability in the expanding geographical distribution of the pest. In the current study, the genetic variability was analysed among geographically confined populations of BSFB from thirty locations of Odisha adopting RAPD-PCR technique. Twenty random 10-mer oligonucleotide primers were cast off to amplify genomes of thirty BSFB populations. A total of 118 bands were spotted by 10 primers, of which 116 were polymorphic. Thirteen unique bands were recognized which will be helpful for developing diagnostic markers. Genetic similarity among BSFB populations ranges from 0.37 to 0.93, with a mean of 0.65 specifying that wide genetic variation showed among BSFB populations at molecular level. Most of the populations could be, especially prominent from each other and clustered into two major clusters. The Structure software indicated a significant genetic differentiation among the populations. More research with host differentials can establish their biotype status.



中文翻译:

印度奥里萨邦茄子果实和嫩芽(BSFB)种群的遗传多样性

在南亚和东亚南部,茄子果实和and虫(BSFB)是对茄子最有害的害虫。已知该害虫分别对芽和果实造成约16%和70%的损害。在有害生物不断扩大的地理分布范围内准确追踪遗传变异具有实际意义。在本研究中,采用RAPD-PCR技术,分析了奥里萨邦30个地区的BSFB地理受限人群的遗传变异。丢弃20个随机的10聚体寡核苷酸引物以扩增30个BSFB群体的基因组。10条引物共发现118条条带,其中116条为多态性。识别出13个独特的条带,这将有助于开发诊断标记。BSFB人群之间的遗传相似性在0.37至0.93之间,平均值为0.65,表明BSFB种群在分子水平上显示出广泛的遗传变异。大多数人口可能彼此之间特别是彼此突出,并且可以分为两个主要群体。结构软件表明种群之间存在显着的遗传分化。有关宿主差异的更多研究可以确定其生物型状态。

更新日期:2020-10-17
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