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Long noncoding RNA LINC00460 promotes the progression of cervical cancer via regulation of the miR-361-3p/Gli1 axis
Human Cell ( IF 4.3 ) Pub Date : 2020-10-16 , DOI: 10.1007/s13577-020-00447-2
Fan Li 1, 2 , Weipei Zhu 1 , Zhijie Wang 2
Affiliation  

Mounting evidence indicates that the long non-coding RNA (lncRNA) LINC00460 plays an oncogenic role in tumor progression; however, the role of LINC00460 in cervical cancer (CC) remains unknown. In this study, we found that LINC00460 was frequently upregulated in CC tissues and cell lines. Knockdown of LINC00460 repressed CC cell growth and invasion in vitro and attenuated tumorigenesis in vivo. Mechanistically, miR-361-3p was predicted as a direct target of LINC00460 by bioinformatics analysis, which was further confirmed by qRT-PCR, dual-luciferase reporter assays, and rescue experiments. Furthermore, miR-361-3p targeted the 3′ untranslated region (UTR) of Gli1 mRNA and repressed its expression. Taken together, our study revealed that LINC00460 functions as an oncogenic lncRNA in CC, indicating the likely participation of the LINC00460/miR-361-3p/Gli1 pathway in the disease. Accordingly, our results provide new insight into CC tumorigenesis.



中文翻译:

长链非编码 RNA LINC00460 通过调节 miR-361-3p/Gli1 轴促进宫颈癌的进展

越来越多的证据表明,长链非编码 RNA (lncRNA) LINC00460 在肿瘤进展中发挥着致癌作用。然而,LINC00460 在宫颈癌 (CC) 中的作用仍然未知。在这项研究中,我们发现 LINC00460 在 CC 组织和细胞系中经常上调。敲除 LINC00460 在体外抑制 CC 细胞生长和侵袭,并在体内减弱肿瘤发生。从机制上讲,通过生物信息学分析,miR-361-3p 被预测为 LINC00460 的直接靶标,并通过 qRT-PCR、双荧光素酶报告基因分析和救援实验进一步证实。此外,miR-361-3p 靶向Gli1的 3' 非翻译区 (UTR)mRNA 并抑制其表达。总之,我们的研究表明 LINC00460 在 CC 中作为致癌 lncRNA 发挥作用,表明 LINC00460/miR-361-3p/Gli1 通路可能参与了该疾病。因此,我们的结果为CC肿瘤发生提供了新的见解。

更新日期:2020-10-17
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