The aim of this research was to optimize bio-succinic acid production from glycerol by using Actinobacillus succinogenes. The effects of different substrate types (pure and crude glycerol), cell patterns (free cells and immobilized cells in agar), and carbonate sources (MgCO₃ and CaCO₃) on succinic (SA) production were studied in serum bottles. The maximum succinic acid concentration of 8.9 ± 0.5 g/L and yield of 1.27 g SA/g GLR were achieved using crude glycerol fermentation by immobilized cells supplemented with10 g/L of MgCO₃ to increase dissolved CO₂ in the system. However, a high concentration of MgCO₃ (20 g/L) resulted in an adverse effect on SA production due to increasing pH which decreased CO₂ fixation in the C4 pathway. The optimal conditions were used for up-scaling SA fermentation in a 1-L bioreactor using batch and semi-continuous operations. Higher SA concentration, glycerol utilization, and SA yield of 10.8 g/L, 88.8%w/w, and 1.25 g SA/g GLR, respectively, were achieved using the batch process. The development process is potent for further up-scaling the study for SA production.

这项研究的目的是通过使用琥珀酸放线杆菌来优化甘油的生物琥珀酸生产。在血清瓶中研究了不同底物类型（纯甘油和粗甘油），细胞模式（琼脂中的游离细胞和固定化细胞）以及碳酸盐来源（MgCO ₃和CaCO ₃）对琥珀酸（SA）生产的影响。使用粗甘油发酵通过固定化细胞补充10 g / L MgCO ₃来增加系统中溶解的CO _2的粗甘油发酵，最大琥珀酸浓度为8.9±0.5 g / L，产率为1.27 g SA / g GLR 。但是，高浓度的MgCO ₃（20 g / L）对pH的升高会导致SA产生不利影响，因为pH升高会降低C4途径中的CO ₂固定。最佳条件用于通过分批和半连续操作在1升生物反应器中扩大SA发酵的规模。使用分批工艺可分别实现更高的SA浓度，甘油利用率和SA产量，分别为10.8 g / L，88.8％w / w和1.25 g SA / g GLR。开发过程对于进一步扩大SA生产的研究非常有效。