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Advances in fluorescent sensors for β-galactosidase
Materials Chemistry Frontiers ( IF 7 ) Pub Date : 2020-10-15 , DOI: 10.1039/d0qm00683a
Meng Li 1, 2, 3, 4, 5 , Mengqing Yang 1, 2, 3, 4, 5 , Wei-Hong Zhu 6, 7, 8, 9, 10
Affiliation  

β-galactosidase (β-Gal), a lysosomal hydrolytic enzyme, plays a significant role in the catalytic hydrolysis of glycosidic bonds, which transforms lactose into galactose. The abnormality of β-Gal in cells is associated with many diseases such as ganglioside deposition, primary ovarian cancer, and also the occurrence of cell senescence. In the past few years, great efforts have been made towards the detection of β-Gal activity in vivo based on fluorescent sensors due to their high sensitivity, selectivity and spatiotemporal resolution imaging. This minireview summarizes the advances in the molecular design strategy and biological applications of fluorescent probes for β-Gal, focusing on detection accuracy enhancement, especially with aggregation-induced emission (AIE) for background minimization and cell permeability promotion. The current challenges and further directions in tracing β-Gal are also discussed.

中文翻译:

β-半乳糖苷酶荧光传感器的研究进展

β-半乳糖苷酶(β-Gal)是一种溶酶体水解酶,在糖苷键的催化水解中起重要作用,糖苷键将乳糖转化为半乳糖。细胞中β-Gal的异常与许多疾病有关,例如神经节苷脂沉积,原发性卵巢癌以及细胞衰老的发生。在过去的几年中,为检测体内β-Gal活性做出了巨大的努力基于荧光传感器,因为它们具有高灵敏度,选择性和时空分辨率成像。这份小型回顾总结了针对β-Gal的荧光探针的分子设计策略和生物学应用方面的进展,重点是提高检测精度,尤其是利用聚集诱导发射(AIE)来实现背景最小化和细胞通透性提升。还讨论了追踪β-Gal的当前挑战和进一步的方向。
更新日期:2020-11-03
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