In the current study, our specific aim was to characterize the Klotho protein and expression levels in the hippocampus and prefrontal cortex of old rats treated with different diets (high-fat, high-protein, low-calorie, high-protein and low-calorie). Rats were treated with high-fat, high-protein, low-calorie, low-calorie high-protein diets for 10 weeks and then behavioral and molecular assessments were evaluated. Statistical analysis showed the percentage of open arm time was increased in the high-protein, low-calorie and low-calorie high-protein groups compared with old control (old-C) rats. The percentage of open arm entries was increased in the low-calorie and low-calorie high-protein group compared with old-C rats. The body weight and serum triglyceride were decreased in the low-calorie and low-calorie high-protein groups in comparison to control old rats. Low-calorie and low-calorie high-protein treatments statistically enhanced caspase-3 level compared with old-C rats in the hippocampus and prefrontal cortex. Treatment of old rats with high-protein, low-calorie and low-calorie high-protein could increase Klotho-α level compared with control old rats. The levels of Klotho-α, c-fos and brain-derived neurotrophic factors were decreased in the low-calorie high-protein group in Klotho inhibitor's presence compared with the low-calorie high-protein group. According to our findings, Klotho-α level was reduced in old rats. Low-calorie, high-protein and particularly low-calorie high-protein diets increased this protein level and consequently increased neuronal plasticity and improved memory function.

中文翻译：

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高蛋白低热量饮食改善大鼠大脑中的抗衰老Klotho蛋白：高脂饮食的毒性作用

在本研究中，我们的具体目的是表征不同饮食（高脂肪、高蛋白、低热量、高蛋白和低热量饮食）老年大鼠海马和前额皮质中的 Klotho 蛋白及其表达水平。 ）。大鼠接受高脂肪、高蛋白、低热量、低热量高蛋白饮食治疗10周，然后进行行为和分子评估。统计分析显示，与老对照（old-C）大鼠相比，高蛋白、低热量和低热量高蛋白组的开臂时间百分比有所增加。与老C组大鼠相比，低热量组和低热量高蛋白组的开臂进入百分比增加。与对照组老年大鼠相比，低热量组和低热量高蛋白组的体重和血清甘油三酯降低。与老C组大鼠相比，低热量和低热量高蛋白治疗在统计学上提高了海马和前额皮质中的caspase-3水平。高蛋白、低热量和低热量高蛋白治疗老年大鼠与对照老年大鼠相比，可增加Klotho-α水平。与低热量高蛋白组相比，Klotho抑制剂存在下低热量高蛋白组的Klotho-α、c-fos和脑源性神经营养因子的水平降低。根据我们的研究结果，老年大鼠中 Klotho-α 水平降低。低热量、高蛋白，特别是低热量高蛋白饮食增加了这种蛋白质水平，从而增加了神经元可塑性并改善了记忆功能。