当前位置: X-MOL 学术Biotechnol. Biofuels › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Comparative transcriptome study of switchgrass (Panicum virgatum L.) homologous autopolyploid and its parental amphidiploid responding to consistent drought stress
Biotechnology for Biofuels ( IF 6.3 ) Pub Date : 2020-10-15 , DOI: 10.1186/s13068-020-01810-z
Peilin Chen 1, 2 , Jing Chen 1 , Min Sun 1 , Haidong Yan 1, 3 , Guangyan Feng 1 , Bingchao Wu 1 , Xinquan Zhang 1 , Xiaoshan Wang 1 , Linkai Huang 1
Affiliation  

Newly formed polyploids may experience short-term adaptative changes in their genome that may enhance the resistance of plants to stress. Considering the increasingly serious effects of drought on biofuel plants, whole genome duplication (WGD) may be an efficient way to proceed with drought resistant breeding. However, the molecular mechanism of drought response before/after WGD remains largely unclear. We found that autoploid switchgrass (Panicum virgatum L.) 8X Alamo had higher drought tolerance than its parent amphidiploid 4X Alamo using physiological tests. RNA and microRNA sequencing at different time points during drought were then conducted on 8X Alamo and 4X Alamo switchgrass. The specific differentially expressed transcripts (DETs) that related to drought stress (DS) in 8X Alamo were enriched in ribonucleoside and ribonucleotide binding, while the drought-related DETs in 4X Alamo were enriched in structural molecule activity. Ploidy-related DETs were primarily associated with signal transduction mechanisms. Weighted gene co-expression network analysis (WGCNA) detected three significant DS-related modules, and their DETs were primarily enriched in biosynthesis process and photosynthesis. A total of 26 differentially expressed microRNAs (DEmiRs) were detected, and among them, sbi-microRNA 399b was only expressed in 8X Alamo. The targets of microRNAs that were responded to polyploidization and drought stress all contained cytochrome P450 and superoxide dismutase genes. This study explored the drought response of 8X and 4X Alamo switchgrass on both physiological and transcriptional levels, and provided experimental and sequencing data basis for a short-term adaptability study and drought-resistant biofuel plant breeding.

中文翻译:

柳枝稷 (Panicum virgatum L.) 同源同源多倍体及其亲本双二倍体响应持续干旱胁迫的比较转录组研究

新形成的多倍体可能会在其基因组中经历短期的适应性变化,这可能会增强植物对压力的抵抗力。考虑到干旱对生物燃料植物的影响日益严重,全基因组复制(WGD)可能是进行抗旱育种的有效方法。然而,WGD前后干旱响应的分子机制仍不清楚。我们通过生理测试发现,同倍体柳枝稷 (Panicum virgatum L.) 8X Alamo 比其亲本双二倍体 4X Alamo 具有更高的耐旱性。然后在 8X Alamo 和 4X Alamo 柳枝稷上进行干旱期间不同时间点的 RNA 和 microRNA 测序。8X Alamo 中与干旱胁迫 (DS) 相关的特异性差异表达转录本 (DET) 富含核糖核苷和核糖核苷酸结合,而 4X Alamo 中与干旱相关的 DET 富含结构分子活性。倍性相关的 DET 主要与信号转导机制有关。加权基因共表达网络分析(WGCNA)检测到三个重要的DS相关模块,它们的DET主要富集在生物合成过程和光合作用中。共检测到 26 个差异表达的 microRNA (DEmiR),其中 sbi-microRNA 399b 仅在 8X Alamo 中表达。响应多倍化和干旱胁迫的 microRNA 的靶标都含有细胞色素 P450 和超氧化物歧化酶基因。本研究探讨了 8X 和 4X 阿拉莫柳枝稷在生理和转录水平上的干旱响应,
更新日期:2020-10-16
down
wechat
bug