This study aimed to investigate the clinicopathological significance and prospective molecular mechanism of RUNX family transcription factor 2 (RUNX2) in lung squamous cell carcinoma (LUSC). The authors used immunohistochemistry (IHC), RNA-seq, and microarray data from multi-platforms to conduct a comprehensive analysis of the clinicopathological significance and molecular mechanism of RUNX2 in the occurrence and development of LUSC. RUNX2 expression was significantly higher in 16 LUSC tissues than in paired non-cancerous tissues detected by IHC ( P < 0.05). RNA-seq data from the combination of TCGA and genotype-tissue expression (GTEx) revealed significantly higher expression of RUNX2 in 502 LUSC samples than in 476 non-cancer samples. The expression of RUNX2 protein was also significantly higher in pathologic T3-T4 than in T1-T2 samples ( P = 0.031). The pooled standardised mean difference (SMD) for RUNX2 was 0.87 (95% CI, 0.58–1.16), including 29 microarrays from GEO and one from ArrayExpress. The co-expression network of RUNX2 revealed complicated connections between RUNX2 and 45 co-expressed genes, which were significantly clustered in pathways including ECM-receptor interaction, focal adhesion, protein digestion and absorption, human papillomavirus infection and PI3K-Akt signalling pathway. Overexpression of RUNX2 plays an essential role in the clinical progression of LUSC.

中文翻译：

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综合表达分析显示肺鳞状细胞癌组织中 RUNX2 上调

本研究旨在探讨RUNX家族转录因子2（RUNX2）在肺鳞状细胞癌（LUSC）中的临床病理意义和前瞻性分子机制。作者利用多平台的免疫组化（IHC）、RNA-seq和微阵列数据，对RUNX2在LUSC发生发展中的临床病理意义和分子机制进行了综合分析。RUNX2 在 16 个 LUSC 组织中的表达显着高于 IHC 检测到的配对非癌组织（ 磷< 0.05)。来自 TCGA 和基因型组织表达 (GTEx) 组合的 RNA-seq 数据显示，502 个 LUSC 样本中 RUNX2 的表达显着高于 476 个非癌症样本。RUNX2 蛋白在病理 T3-T4 中的表达也显着高于 T1-T2 样本（ 磷= 0.031)。RUNX2 的汇总标准化平均差 (SMD) 为 0.87 (95% CI, 0.58–1.16)，包括来自 GEO 的 29 个微阵列和一个来自 ArrayExpress 的微阵列。RUNX2的共表达网络揭示了RUNX2与45个共表达基因之间的复杂联系，这些基因显着聚集在ECM-受体相互作用、粘着斑、蛋白质消化吸收、人乳头瘤病毒感染和PI3K-Akt信号通路等通路中。RUNX2 的过表达在 LUSC 的临床进展中起重要作用。