Natural Product Communications ( IF 1.8 ) Pub Date : 2020-10-15 , DOI: 10.1177/1934578x20965465 Hye-Sun Lim 1 , Yoonju Kim 1 , Yu Jin Kim 1, 2 , Eunjin Sohn 1 , Joo-Hwan Kim 3 , Soo-Jin Jeong 1
Crinum asiaticum var. japonicum Baker is a Korean herbal medicine that is traditionally prescribed for reducing fever and inflammation. In the present study, we investigated if the ethanol extract of C. asiaticum seeds (ECAS) influences the hallmarks of Alzheimer’s disease (AD) pathogenesis. ECAS markedly inhibited the activity of acetylcholinesterase (AChE). Concurrent treatment with hydrogen peroxide (H2O2) and ECAS significantly prevented the neuronal cell death by regulating phosphorylation of cyclic adenosine monophosphate response element-binding protein and p38 mitogen-activated protein kinase. ECAS revealed antineuroinflammatory effects by inhibiting nitric oxide production and suppressing inducible nitric oxide expression in lipopolysaccharide-stimulated BV-2 microglia. Furthermore, the high-performance liquid chromatographic analysis determined lycorine as a standard compound of ECAS. Our data suggest that ECAS has inhibitory effects on AD pathogenesis such as AChE activation, neuronal damage, and neuroinflammation.
中文翻译:
的影响文殊兰变种。日本面包种子对神经元细胞系的神经保护和抗神经炎症的作用
crinum asiaticum var。japonicum Baker是韩国草药,传统上可减少发烧和发炎。在本研究中,我们调查了亚洲念珠菌种子(ECAS)的乙醇提取物是否影响阿尔茨海默氏病(AD)发病机理的标志。ECAS显着抑制乙酰胆碱酯酶(AChE)的活性。同时用过氧化氢(H 2 O 2)和ECAS通过调节环状单磷酸腺苷反应元件结合蛋白和p38丝裂原激活的蛋白激酶的磷酸化,显着预防神经元细胞死亡。ECAS通过抑制一氧化氮的产生并抑制脂多糖刺激的BV-2小胶质细胞中可诱导的一氧化氮的表达,揭示了抗神经炎作用。此外,高效液相色谱分析确定了赖氨酸为ECAS的标准化合物。我们的数据表明,ECAS对AD发病机制具有抑制作用,例如AChE激活,神经元损伤和神经炎症。