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3D brain tissue physiological model with co-cultured primary neurons and glial cells in hydrogels
Journal of Tissue Engineering ( IF 8.2 ) Pub Date : 2020-10-14 , DOI: 10.1177/2041731420963981
Ilaria Raimondi 1 , Marta Tunesi 1 , Gianluigi Forloni 2 , Diego Albani 2 , Carmen Giordano 1
Affiliation  

Recently, researchers have focused on the role of gut microbiota on human health and reported the existence of a bidirectional relationship between intestinal microbiota and the brain, referred to as microbiota-gut-brain axis (MGBA). In this context, the development of an organ-on-a-chip platform recapitulating the main players of the MGBA would help in the investigations of the biochemical mechanisms involved. In this work, we focused on the development of a new, hydrogel-based, 3D brain-like tissue model to be hosted in the brain compartment of the aforementioned platform. We previously cultured primary mouse microglial cells, cortical neurons and astrocytes independently, once embedded or covered by a millimeter layer of two selected collagen-based hydrogels. We evaluated cell metabolic activity up to 21 days, cell morphology, spatial distribution and synapse formation. Then, we exploited the best performing culturing condition and developed a more complex brain-like tissue model based on the co-culture of cortical neurons and glial cells in physiological conditions. The obtained results indicate that our 3D hydrogel-based brain tissue model is suitable to recapitulate in vitro the key biochemical parameters of brain tissue.



中文翻译:

水凝胶中与原代神经元和神经胶质细胞共培养的3D脑组织生理模型

最近,研究人员集中研究了肠道菌群在人类健康中的作用,并报道了肠道菌群与大脑之间存在双向关系,即微生物菌群-肠脑轴(MGBA)。在这种情况下,重现MGBA主要参与者的芯片上器官平台的开发将有助于调查涉及的生化机制。在这项工作中,我们专注于开发新的基于水凝胶的3D脑样组织模型,该模型将托管在上述平台的脑室中。一旦嵌入或被两种选定的基于胶原蛋白的水凝胶的毫米层覆盖,我们以前就分别培养了原代小鼠小神经胶质细胞,皮质神经元和星形胶质细胞。我们评估了长达21天的细胞代谢活性,细胞形态,空间分布和突触形成。然后,我们利用表现最佳的培养条件,并在生理条件下基于皮层神经元和神经胶质细胞的共培养,建立了更复杂的脑样组织模型。获得的结果表明,我们基于3D水凝胶的脑组织模型适合于体外概括脑组织的关键生化参数。

更新日期:2020-10-16
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