Four cationic heteroleptic iridium(III) complexes containing a 2,2′-bipyridine (bpy) ligand with one or two tetraethylene glycol (TEG) groups attached in the 4 or 4,4′ positions were synthesized to create new water-soluble electrogenerated chemiluminescence (ECL) luminophores bearing a convenient point of attachment for the development of ECL-labels. The novel TEG-derivatized bipyridines were incorporated into [Ir(C^∧N)₂(R-bpy-R′)]Cl complexes, where C^∧N = 2-phenylpyridine anion (ppy) or 2-phenylbenzo[d]thiazole anion (bt), through reaction with commercially available ([Ir(C^∧N)₂(μ-Cl)]₂ dimers. The novel [Ir(C^∧N)₂(Me-bpy-TEG)]Cl and [Ir(C^∧N)₂(TEG-bpy-TEG)]Cl complexes in aqueous solution largely retained the redox potentials and emission spectra of the parent [Ir(C^∧N)₂(Me-bpy-Me)]PF₆ (where Me-bpy-Me = 4,4′methyl-2,2′-bipyridine) luminophores in acetonitrile, and exhibited ECL intensities similar to those of [Ru(bpy)₃]²⁺ and the analogous [Ir(C^∧N)₂(pt-TEG]Cl complexes (where pt-TEG = 1-(TEG)-4-(2-pyridyl)-1,2,3-triazole). These complexes can be readily adapted for bioconjugation and considering the spectral distributions of [Ir(ppy)₂(Me-bpy-TEG)]⁺ and [Ir(ppy)₂(pt-TEG)]⁺, show a viable strategy to create ECL-labels with different emission colors from the same commercial [Ir(ppy)₂(μ-Cl)]₂ precursor.

合成了四个阳离子杂合铱（III）配合物，它们包含一个2,2'-联吡啶（bpy）配体，并在4或4,4'位置连接一个或两个四甘醇（TEG）基团，以创建新的水溶性电致化学发光（ECL）发光体带有一个方便的连接点，可用于开发ECL标签。将新型TEG衍生的联吡啶掺入[Ir（^C∧N）₂（R-bpy-R'）] Cl络合物中，其中^C∧N = 2-苯基吡啶阴离子（ppy）或2-苯基苯并[d]噻唑阴离子（bt），通过与市售的[[Ir（^C∧N）₂（μ-Cl）] ₂二聚体反应。新型[Ir（^C∧N）₂（ME-BPY-TEG）] Cl和物[Ir（C ^∧ N）₂（TEG-BPY-TEG）]的水溶液氯配合物基本上保留了亲本的[Ir（C的氧化还原电位和发射光谱^∧ N）₂（Me-bpy-Me）] PF ₆（其中Me-bpy-Me = 4,4'methyl-2,2'-联吡啶）发光体在乙腈中，并且其ECL强度与[Ru（bpy）₃ ]相似²⁺和类似的[Ir（^C∧N）₂（pt-TEG] Cl络合物（其中pt-TEG = 1-（TEG）-4-（2-吡啶基）-1,2,3-三唑）。配合物可以很容易地用于生物共轭，并考虑[Ir（ppy）₂（Me-bpy-TEG）] ⁺和[Ir（ppy）的光谱分布_图2（pt-TEG）] ⁺显示了一种可行的策略，可以从同一市售[Ir（ppy）₂（μ-Cl）] ₂前体创建具有不同发射颜色的ECL标签。