Increasing the growth rate of the industrial host Corynebacterium glutamicum is a promising target to rise productivities of growth coupled product formation. As a prerequisite, detailed knowledge about the tight regulation network is necessary for identifying promising metabolic engineering goals. Here, we present comprehensive metabolic and transcriptional analysis of C. glutamicum ATCC 13032 growing under glucose limited chemostat conditions with μ = 0.2, 0.3, and 0.4 h–1. Intermediates of central metabolism mostly showed rising pool sizes with increasing growth. 13C-metabolic flux analysis (13C-MFA) underlined the fundamental role of central metabolism for the supply of precursors, redox, and energy equivalents. Global, growth-associated, concerted transcriptional patterns were not detected giving rise to the conclusion that glycolysis, pentose-phosphate pathway, and citric acid cycle are predominately metabolically controlled under glucose-limiting chemostat conditions. However, evidence is found that transcriptional regulation takes control over glycolysis once glucose-rich growth conditions are installed.

中文翻译：

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在葡萄糖限制的恒化条件下重新审视谷氨酸棒杆菌的生长模量

提高工业宿主谷氨酸棒杆菌的生长速率是提高生长耦合产物形成生产率的有希望的目标。作为先决条件，关于严密调控网络的详细知识对于确定有希望的代谢工程目标是必要的。在这里，我们展示了谷氨酸棒杆菌 ATCC 13032 在葡萄糖限制的恒化器条件下生长的综合代谢和转录分析，μ = 0.2、0.3 和 0.4 h-1。中央代谢的中间体大多显示出随着生长的增加而增加的池大小。13C-代谢通量分析 (13C-MFA) 强调了中央代谢对前体、氧化还原和能量等价物供应的基本作用。未检测到全球性的、与生长相关的、协同的转录模式，从而得出以下结论：糖酵解、戊糖-磷酸途径和柠檬酸循环在葡萄糖限制的恒化条件下主要受代谢控制。然而，有证据表明，一旦安装了富含葡萄糖的生长条件，转录调节就会控制糖酵解。