Abstract Introduction Sunset Yellow FCF (SY), used frequently in ready-made foods, cosmetics, and the pharmaceutical industry, may cause many health problems. This study is intended to evaluate the morphological and cellular effects of SY on the embryonic chicken immune system throughout incubation. Material and Methods Babcock white leghorn eggs were randomly divided into four groups. Besides a control group, there were three treatment groups which received a single injection of 200, 1,000, or 2,000 ng of SY into the air sac just before incubation. The eggs were opened on the 10th, 13th, 16th, and 21st days of incubation. Samples of the bursa of Fabricius, thymus, and spleen were taken from embryos. Serial sections of 5 μm thickness were stained with histological methods and routine histological procedures were performed. Results An increase in the spleen volume was determined as the hatching time of the chicks approached. The highest eosinophil ratio was found in the SY1,000 and SY2,000 groups (P < 0.05), where the most significant change was developmental retardation in the thymus. In the bursa of Fabricius, there was less lymphocyte accumulation and eosinophilic cell infiltration with increasing doses. Conclusion It was concluded that in ovo administered SY has undesired effects on embryonic development of the bursa of Fabricius, spleen, and thymus, and on spleen volume.

中文翻译：

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不同鸡胚时期日落黄FCF对免疫系统器官的影响

摘要 简介 日落黄 FCF (SY) 经常用于现成食品、化妆品和制药行业，可能会导致许多健康问题。本研究旨在评估 SY 在整个孵化过程中对胚胎鸡免疫系统的形态和细胞影响。材料与方法 巴布科克白来河角鸡蛋随机分为四组。除对照组外，还有三个治疗组在孵化前将 200、1,000 或 2,000 ng SY 单次注射到气囊中。在孵化的第 10 天、第 13 天、第 16 天和第 21 天打开鸡蛋。法氏囊、胸腺和脾脏的样本取自胚胎。用组织学方法对 5 μm 厚的连续切片进行染色，并进行常规组织学程序。结果随着雏鸡孵化时间的临近，脾脏体积增加。SY1,000 和 SY2,000 组的嗜酸性粒细胞比率最高（P < 0.05），其中最显着的变化是胸腺发育迟缓。在法氏囊中，随着剂量的增加，淋巴细胞聚集和嗜酸性细胞浸润减少。结论 得出的结论是，卵内施用的 SY 对法氏囊、脾脏和胸腺的胚胎发育以及脾脏体积具有不良影响。随着剂量的增加，淋巴细胞聚集和嗜酸性细胞浸润减少。结论 得出的结论是，卵内施用的 SY 对法氏囊、脾脏和胸腺的胚胎发育以及脾脏体积具有不良影响。随着剂量的增加，淋巴细胞聚集和嗜酸性细胞浸润减少。结论 得出的结论是，卵内施用的 SY 对法氏囊、脾脏和胸腺的胚胎发育以及脾脏体积具有不良影响。