ABSTRACT

Circular RNAs (circRNAs) are a class of widely expressed noncoding RNA with significant regulatory potential discovered in recent years. The purpose of this study was to investigate the effects of hsa_circ_0001785 on the proliferation, migration and invasion of breast cancer (BC) cells in vivo and in vitro and the potential underlying molecular mechanism. In the present study, the expressions of hsa_circ_0001785 in five BC cells (T47D, MCF-7, MDA-MB-453, MDA-MB-231 and BT-549) and one normal breast cell (MCF-10A) were the first to examined by qRT-PCR. Then, we studied the biological function of hsa_circ_0001785 in BC by in vivo and in vitro experiments. CCK-8, clone formation, wound-healing and Transwell assays were performed to analyze the cellular proliferation, migration and invasion in vitro. The subcutaneous tumor model of nude mice was used for in vivo experiment. In addition, we determined that hsa_circ_0001785 acted as competing endogenous RNAs (ceRNAs) in BC by RNA immunoprecipitation (RIP) and dual-luciferase reporter assays. Results showed that the expressions of hsa_circ_0001785 were decreased in BC cells. Hsa_circ_0001785 overexpression inhibited the proliferation, migration, invasion of BC cells and tumor growth in nude mice. RIP and dual-luciferase reporter assay demonstrated that hsa_circ_0001785 could regulate the SOCS3 by sponging miR-942. In general, circular RNA hsa_circ_0001785 inhibits the proliferation, migration and invasion of BC cells by modulating the miR-942/SOCS3 signaling axis.

摘要

环状RNA（circRNA）是一类广泛表达的非编码RNA，近年来发现具有显着的调控潜力。本研究的目的是调查的hsa_circ_0001785对乳腺癌（BC）的细胞的增殖，迁移和侵袭的影响在体内和在体外和分子机制的潜力。在本研究中，hsa_circ_0001785 在 5 种 BC 细胞（T47D、MCF-7、MDA-MB-453、MDA-MB-231 和 BT-549）和一种正常乳腺细胞（MCF-10A）中的表达最先出现。通过 qRT-PCR 检查。然后，我们研究了通过在BC hsa_circ_0001785的生物学功能体内和体外实验。进行CCK-8、克隆形成、伤口愈合和Transwell测定以分析体外细胞增殖、迁移和侵袭。裸鼠皮下肿瘤模型用于体内实验。此外，我们通过 RNA 免疫沉淀 (RIP) 和双荧光素酶报告基因测定确定 hsa_circ_0001785 在 BC 中充当竞争性内源性 RNA (ceRNA)。结果表明，hsa_circ_0001785在BC细胞中的表达降低。Hsa_circ_0001785过表达抑制裸鼠BC细胞的增殖、迁移、侵袭和肿瘤生长。RIP 和双荧光素酶报告基因检测表明 hsa_circ_0001785 可以通过海绵 miR-942 调节 SOCS3。一般而言，circRNA hsa_circ_0001785 通过调节 miR-942/SOCS3 信号轴来抑制 BC 细胞的增殖、迁移和侵袭。