ABSTRACT

Nature’s repertoire of bio-halogenase enzymes is intriguing with halogenases from various natural product biosynthetic clusters that carry out site and region-specific halogenation of diverse bioactive precursors and molecules. Currently, we have a comprehensive catalogue of cryptic and non-cryptic halogenases that act on simple to complex aliphatic and aromatic molecular scaffolds. This will open up further synthetic and biosynthetic opportunities for C-H activation, ring formation and functionalization of different molecular structures. In fact, halogenases were exploited over the years for these potential applications, to replace traditional chemical halogenation chemistries toward creating economical and environmentally benign methodologies and also for biosynthetic pathways. This review will discuss our advances in utilizing bio-halogenases to generate both in vivo and in vitro biosynthetic pathways; summarizing all naturals and non-naturals that are synthesized with a direct bio-halogenase incorporation.

摘要

自然界的生物卤化酶库对来自各种天然产物生物合成簇的卤化酶很感兴趣，这些簇对不同的生物活性前体和分子进行位点和区域特异性卤化。目前，我们有一个全面的隐蔽和非隐蔽卤化酶目录，它们作用于简单到复杂的脂肪族和芳香族分子支架。这将为不同分子结构的 CH 活化、环形成和功能化开辟进一步的合成和生物合成机会。事实上，卤化酶多年来一直被用于这些潜在的应用，以取代传统的化学卤化化学，以创造经济和环境友好的方法，也用于生物合成途径。体内和体外生物合成途径；总结了通过直接生物卤化酶掺入合成的所有天然和非天然物质。