Lipase was extracted from germinating seeds of Helianthus annus (Sunflower), Zea mays (Maize), and Brassica compastris (Mustard). The lipolytic activity was assessed using olive oil as substrate at different germination‐time and the maximum‐activity was obtained after 120 hr. Partial‐purification was executed by precipitating the seed‐homogenate with varying concentration of ammonium sulfate solution. 80% ammonium sulfate solution showed maximum lipase activity of 5320IUml⁻¹, 3500IUml⁻¹, 3080IUml⁻¹ with 9.6, 6.9, and 4.8‐fold purification and total protein content of 162, 84, and 60 mg for partially purified enzyme extracts namely SN5, BN5, and MN5, respectively. The optimum temperature and pH observed for hydrolysis of olive oil were 37°C, and 8.0 respectively. Enzyme was found to be stable upto 6 days at 4°C and its activity was stimulated by Ca⁺²ions. Oil‐stains removal from cotton fabric was observed to be superior in the presence of lipase and detergent. Moreover, the SN5, BN5, and MN5 lipase increased free fatty acid release upto 4.2, 4.3, and 3.8 mg, respectively than wastewater without treatment of lipase (0.21 mg) and promoted fat hydrolysis to approximately 40, 42, and 48% mass reduction after 6 hr incubation of fat particle at a concentration of 20 mg/ml. Biodiesel produced by catalyzing transesterification of vegetable oil with SN5, BN5, and MN5 lipase provided an acid value of 0.8, 1.08, and 0.5 mg/g, viscosity 5.50, 5.7, and 5.53 mm²/s and density 0.87, 0.88, and 0.79 g/ml, respectively. To the best of our knowledge, no such study has been conducted prior on lipase from the seeds mentioned above in Azad Kashmir region.

中文翻译：

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回收：从当地生产的食用油种子中部分纯化和鉴定脂肪酶及其在工业中的相关性

脂肪酶是从向日葵（向日葵），玉米（玉米）和芸苔（芥菜）的发芽种子中提取的。使用橄榄油作为底物在不同的发芽时间评估其脂解活性，并在120小时后获得最大活性。通过用不同浓度的硫酸铵溶液沉淀均质种子来进行部分纯化。80％的硫酸铵溶液显示5320IUml的最大脂酶活性^-1，3500IUml ^-1，3080IUml ^-1对于部分纯化的酶提取物（即SN5，BN5和MN5）的9.6、6.9和4.8倍纯化和总蛋白质含量分别为162、84和60 mg。观察到的水解橄榄油的最佳温度和pH分别为37°C和8.0。发现该酶在4°C下最多可稳定6天，并且其活性受到Ca ^+2的刺激离子。在脂肪酶和清洁剂的存在下，棉织物上的油污去除效果更好。此外，SN5，BN5和MN5脂肪酶比未处理脂肪酶的废水（0.21 mg）分别将游离脂肪酸释放增加至4.2、4.3和3.8 mg，并促进脂肪水解减少了40％，42％和48％的质量在以20 mg / ml的浓度孵育脂肪颗粒6小时后。通过用SN5，BN5和MN5脂肪酶催化植物油的酯交换反应生产的生物柴油提供的酸值为0.8、1.08和0.5 mg / g，粘度为5.50、5.7和5.53 mm ² / s，密度为0.87、0.88和0.79克/毫升。据我们所知，在Azad克什米尔地区，从未对上述种子中的脂肪酶进行过此类研究。