Platelet lysate (PL) has been shown to induce chondrogenic differentiation of human umbilical cord‐derived mesenchymal stem cells (hUCMSCs). However, the underlying mechanism is still not clear. The aim of this study was to investigate whether long noncoding RNA H19 is involved in this process. hUCMSCs were isolated, identified and cultured in 5% PL‐supplemented chondrogenic differentiation medium. Chondrogenic differentiation was assessed by Alcian blue staining. The expressions of H19, miR‐29b‐3p, SRY‐related high‐mobility‐group box 9 (SOX9), collagen II and aggrecan were determined by quantitative real‐time PCR and western blot. The interaction between miR‐29b‐3p and H19 or SOX9 was analyzed by luciferase reporter assay. During PL‐induced chondrogenic differentiation of hUCMSCs, expressions of H19 and SOX9 were increased, whereas miR‐29b‐3p expression was decreased. H19 overexpression promoted, whereas H19 silencing attenuated the PL‐induced chondrogenic differentiation of hUCMSCs. SOX9 was identified as a direct target of miR‐29b‐3p, and H19 was observed to act as a sponge of miR‐29b‐3p to up‐regulate SOX9 expression. The chondrogenic differentiation‐promoting effect of H19 overexpression was negated when miR‐29b‐3p expression was up‐regulated by Lenti‐miR‐29b‐3p infection. In conclusion, PL induced chondrogenic differentiation of hUCMSCs by regulating the H19/miR‐29b‐3p/SOX9 axis.

中文翻译：

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血小板裂解液通过调节 lncRNA H19/miR-29b-3p/SOX9 轴诱导脐带间充质干细胞的软骨分化

血小板裂解物 (PL) 已被证明可诱导人脐带间充质干细胞 (hUCMSCs) 的软骨形成分化。然而，其潜在机制仍不清楚。本研究的目的是调查长链非编码 RNA H19 是否参与了这一过程。在 5% PL 补充的软骨分化培养基中分离、鉴定和培养 hUCMSCs。通过阿尔辛蓝染色评估软骨形成分化。通过实时定量 PCR 和蛋白质印迹法测定 H19、miR-29b-3p、SRY 相关高迁移率组盒 9（SOX9）、II 型胶原和蛋白聚糖的表达。通过荧光素酶报告基因测定分析 miR-29b-3p 与 H19 或 SOX9 之间的相互作用。在 PL 诱导的 hUCMSCs 软骨分化过程中，H19 和 SOX9 的表达增加，而 miR-29b-3p 表达降低。H19 过表达促进，而 H19 沉默减弱了 PL 诱导的 hUCMSCs 软骨分化。SOX9 被确定为 miR-29b-3p 的直接靶标，并且观察到 H19 作为 miR-29b-3p 的海绵来上调 SOX9 表达。当 Lenti-miR-29b-3p 感染上调 miR-29b-3p 表达时，H19 过表达的软骨形成促进作用被抵消。总之，PL通过调节H19/miR-29b-3p/SOX9轴诱导hUCMSCs的软骨分化。当 Lenti-miR-29b-3p 感染上调 miR-29b-3p 表达时，H19 过表达的软骨形成促进作用被抵消。总之，PL通过调节H19/miR-29b-3p/SOX9轴诱导hUCMSCs的软骨分化。当 Lenti-miR-29b-3p 感染上调 miR-29b-3p 表达时，H19 过表达的软骨形成促进作用被抵消。总之，PL通过调节H19/miR-29b-3p/SOX9轴诱导hUCMSCs的软骨分化。