当前位置: X-MOL 学术Structure › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
The Architecture of Inactivated SARS-CoV-2 with Postfusion Spikes Revealed by Cryo-EM and Cryo-ET
Structure ( IF 5.7 ) Pub Date : 2020-10-15 , DOI: 10.1016/j.str.2020.10.001
Chuang Liu , Luiza Mendonça , Yang Yang , Yuanzhu Gao , Chenguang Shen , Jiwei Liu , Tao Ni , Bin Ju , Congcong Liu , Xian Tang , Jinli Wei , Xiaomin Ma , Yanan Zhu , Weilong Liu , Shuman Xu , Yingxia Liu , Jing Yuan , Jing Wu , Zheng Liu , Zheng Zhang , Lei Liu , Peiyi Wang , Peijun Zhang

The ongoing global pandemic of coronavirus disease 2019 (COVID-19) resulted from the outbreak of SARS-CoV-2 in December 2019. Currently, multiple efforts are being made to rapidly develop vaccines and treatments to fight COVID-19. Current vaccine candidates use inactivated SARS-CoV-2 viruses; therefore, it is important to understand the architecture of inactivated SARS-CoV-2. We have genetically and structurally characterized β-propiolactone-inactivated viruses from a propagated and purified clinical strain of SARS-CoV-2. We observed that the virus particles are roughly spherical or moderately pleiomorphic. Although a small fraction of prefusion spikes are found, most spikes appear nail shaped, thus resembling a postfusion state, where the S1 protein of the spike has disassociated from S2. Cryoelectron tomography and subtomogram averaging of these spikes yielded a density map that closely matches the overall structure of the SARS-CoV postfusion spike and its corresponding glycosylation site. Our findings have major implications for SARS-CoV-2 vaccine design, especially those using inactivated viruses.



中文翻译:

Cryo-EM和Cryo-ET揭示了具有融合后尖峰的灭活SARS-CoV-2的体系结构

2019年12月爆发的SARS-CoV-2导致2019年全球冠状病毒疾病(COVID-19)持续流行。目前,人们正在为快速研发抗COVID-19的疫苗和疗法做出多种努力。目前的候选疫苗使用灭活的SARS-CoV-2病毒;因此,了解灭活SARS-CoV-2的体系结构很重要。我们已经从SARS-CoV-2的繁殖和纯化临床菌株中遗传和结构表征了β-丙内酯灭活病毒。我们观察到病毒颗粒大致呈球形或中等多形。尽管发现了融合前刺突的一小部分,但大多数刺突呈钉状,因此类似于融合后状态,其中刺突的S1蛋白已与S2分离。这些尖峰的冷冻电子断层扫描和子断层图平均得出的密度图与SARS-CoV融合后尖峰的整体结构及其相应的糖基化位点紧密匹配。我们的发现对SARS-CoV-2疫苗的设计具有重要意义,尤其是使用灭活病毒的疫苗。

更新日期:2020-11-03
down
wechat
bug