Urinary tract infections are second most important diseases worldwide due to the increased amount of antibiotic resistant microbes. Among the Gram negative bacteria, P. mirabilis is the dominant biofilm producer in urinary tract infections next to E. coli. Biofilm is a process that produced self-matrix of more virulence pathogens on colloidal surfaces. Based on the above fact, this study was concentrated to inhibit the P. mirabilis biofilm formation by various in-vitro experiments. In the current study, the anti-biofilm effect of essential oils was recovered from the medicinal plant of Solanum nigrum, and confirmed the available essential oils by liquid chromatography-mass spectroscopy analysis. The excellent anti-microbial activity and minimum biofilm inhibition concentration of the essential oils against P. mirabilis was indicated at 200 µg/mL. The absence of viability and altered exopolysaccharide structure of treated cells were showed by biofilm metabolic assay and phenol–sulphuric acid method. The fluorescence differentiation of P. mirabilis treated cells was showed with more damages by confocal laser scanning electron microscope. Further, more morphological changes of essential oils treated cells were differentiated from normal cells by scanning electron microscope. Altogether, the results were reported that the S. nigrum essential oils have anti-biofilm ability.

由于抗生素抗性微生物数量的增加，泌尿道感染是全球第二重要的疾病。在革兰氏阴性细菌中，P。mirabilis是尿道感染中仅次于大肠杆菌的主要生物膜生产者。生物膜是在胶体表面上产生更多毒力病原体自身基质的过程。基于上述事实，本研究通过各种体外实验集中于抑制奇异假单胞菌生物膜的形成。在当前的研究中，精油的抗生物膜作用是从龙葵（Solanum nigrum）的药用植物中回收的，并通过液相色谱-质谱分析确定了可用的精油。精油对奇异假单胞菌的优异的抗菌活性和最小的生物膜抑制浓度显示为200 µg / mL。通过生物膜代谢测定法和苯酚-硫酸法显示处理后的细胞不存在活力和胞外多糖结构改变。共聚焦激光扫描电镜观察发现，P。mirabilis处理的细胞的荧光分化损伤更大。此外，通过扫描电子显微镜将精油处理的细胞的形态变化与正常细胞相区别。总体而言，结果被报道为黑曲霉 香精油具有抗生物膜的能力。