Food producing animals harbouring bacteria carrying drug resistance genes especially the metallo-beta-lactamase (MBL) pose high risk for the human population. In addition, formation of biofilm by these drug resistant pathogens represents major threat to food safety and public health. In this study, metallo-β-lactamases (MβLs) producing Pseudomonas spp. from camel meat were isolated and assessed for their biofilm formation. Further, in vitro and in silico studies were performed to study the effect of flavone naringin on biofilm formation against isolated Pseudomonas spp. A total of 55% isolates were found to produce metallo-β-lactamase enzyme. Naringin mitigated biofilm formation of Pseudomonas isolates up to 57%. Disturbed biofilm architecture and reduced the colonization of bacteria on glass was observed under scanning electron microscope (SEM) and confocal laser scanning microscope (CLSM). The biofilm related traits such as exopolysaccharides (EPS) and alginate production was also reduced remarkably in the presence of naringin. Eradication of preformed biofilms (32–60%) was also observed at the respective 0.50 × MICs. Molecular docking revealed that naringin showed strong affinity towards docked proteins with binding energy ranging from −8.6 to −8.8 kcal mol⁻¹. Presence of metallo-β-lactamase producers indicates that camel meat could be possible reservoir of drug-resistant Pseudomonas species of clinical importance. Naringin was successful in inhibiting biofilm formation as well as eradicating the preformed biofilms and demonstrated strong binding affinity towards biofilm associated protein. Thus, it is envisaged that naringin could be exploited as food preservative especially against the biofilm forming food-borne Pseudomonas species and is a promising prospect for the treatment of biofilm based infections.

携带有耐药基因，尤其是金属β-内酰胺酶（MBL）的细菌的食用动物对人类构成高风险。此外，由这些抗药性病原体形成的生物膜对食品安全和公共卫生构成了重大威胁。在这项研究中，金属β-内酰胺酶（MβLs）产生假单胞菌。从骆驼肉中分离出并评估其生物膜形成。此外，进行了体外和计算机模拟研究，以研究黄酮柚皮苷对分离的假单胞菌属物种生物膜形成的影响。发现总共55％的分离物产生金属β-内酰胺酶。柚皮苷减轻假单胞菌生物膜形成隔离率高达57％。在扫描电子显微镜（SEM）和共聚焦激光扫描显微镜（CLSM）下观察到了扰乱的生物膜结构并减少了细菌在玻璃上的定殖。在柚皮苷的存在下，与生物膜相关的特征，如胞外多糖（EPS）和藻酸盐的产生也显着降低。在各个0.50×MIC处也观察到根除预先形成的生物膜（32–60％）。分子对接显示，柚皮苷对对接蛋白表现出很强的亲和力，结合能范围为-8.6至-8.8 kcal mol ^-1。金属β-内酰胺酶生产者的存在表明骆驼肉可能是耐药性假单胞菌的储藏库具有临床重要性的物种。柚皮苷成功地抑制了生物膜的形成并消除了预先形成的生物膜，并证明了对生物膜相关蛋白的强结合亲和力。因此，设想柚皮苷可以用作食品防腐剂，特别是针对形成生物膜的食源性假单胞菌属物种，并且是治疗基于生物膜的感染的有希望的前景。