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Arabidopsis NHX5 and NHX6 regulate PIN6-mediated auxin homeostasis and growth
Journal of Plant Physiology ( IF 4.3 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.jplph.2020.153305
Shasha Lv , Lu Wang , Xiao Zhang , Xiaojiao Li , Ligang Fan , Yanli Xu , Yingjia Zhao , Huichun Xie , Megan G. Sawchuk , Enrico Scarpella , Quan-Sheng Qiu

NHX5 and NHX6, endosomal Na+,K+/H+ antiporters in Arabidopsis thaliana, play a vital role in growth and development. Our previous study has shown that NHX5 and NHX6 function as H+ leak to regulate auxin-mediated growth in Arabidopsis. In this report, we investigated the function of NHX5 and NHX6 in controlling PIN6-mediated auxin homeostasis and growth in Arabidopsis. Phenotypic analyses found that NHX5 and NHX6 were critical for the function of PIN6, an auxin transporter. We further showed that PIN6 depended on NHX5 and NHX6 in regulating auxin homeostasis. NHX5 and NHX6 were colocalized with PIN6, but they did not interact physically. The conserved acidic residues that are vital for the activity of NHX5 and NHX6 were critical for PIN6 function. Together, NHX5 and NHX6 may regulate PIN6 function by their transport activity.

中文翻译:

拟南芥 NHX5 和 NHX6 调节 PIN6 介导的生长素稳态和生长

NHX5 和 NHX6 是拟南芥中的内体 Na+、K+/H+ 逆向转运蛋白,在生长和发育中起着至关重要的作用。我们之前的研究表明,NHX5 和 NHX6 充当 H+ 泄漏以调节拟南芥中生长素介导的生长。在本报告中,我们研究了 NHX5 和 NHX6 在控制拟南芥中 PIN6 介导的生长素稳态和生长方面的功能。表型分析发现 NHX5 和 NHX6 对生长素转运蛋白 PIN6 的功能至关重要。我们进一步表明 PIN6 依赖于 NHX5 和 NHX6 来调节生长素稳态。NHX5 和 NHX6 与 PIN6 共定位,但它们没有物理相互作用。对 NHX5 和 NHX6 活性至关重要的保守酸性残基对 PIN6 功能至关重要。NHX5 和 NHX6 可以一起通过它们的运输活动来调节 PIN6 的功能。
更新日期:2020-12-01
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