Abstract Dolomitic limestones are carbonate rocks with an inherent high intrinsic bioreceptivity, being especially prone to microbial colonization. Nonetheless, fungal dolomitic limestone bioreceptivity and biodeterioration still remains scarcely characterized. As such, the aims of this work were to: evaluate the biodeteriorative abilities of previously isolated fungi thriving at the Old Cathedral of Coimbra; access fungal limestone bioreceptivity in a laboratory simulated oligotrophic and copiotrophic environment; and to characterize the biodeterioration impacts of these organisms. The obtained results allowed to determine that a significant number of the tested species displayed in vitro biodeteriorative abilities. Inoculation of these species in dolomitic limestone replicas for one year revealed that, with a few exceptions, fungal proliferation was slightly superior in oligotrophic rather than copiotrophic conditions and that, in general, Penicillium chrysogenum, Periconia byssoides and Parengyodontium album displayed the highest average colonization rates. Moreover, most of the tested species produced noticeable alterations in the form of mineral disaggregation, displacement and possible dissolution. The obtained results also allowed to detect calcite mineralization in or near some species hyphae, suggesting that after one-year fungal stone diagenesis and secondary biomineralization was already occurring. This study contributed to further elucidate the nefarious effect resulting from fungal proliferation on dolomitic limestone.

中文翻译：

---

真菌和白云质石灰石相互作用的体外分析：生物接受性和生物退化评估

摘要 白云质灰岩是碳酸盐岩，具有固有的高内在生物接受性，特别容易发生微生物定植。尽管如此，真菌白云质石灰石的生物接受性和生物退化仍然很少被表征。因此，这项工作的目的是： 评估先前在科英布拉旧大教堂繁盛的分离真菌的生物降解能力；在实验室模拟的贫营养和营养环境中获得真菌石灰石的生物接受性；并表征这些生物的生物退化影响。获得的结果允许确定大量的测试物种显示出体外生物降解能力。在白云质石灰岩仿制品中接种这些物种一年后发现，除了少数例外，真菌增殖在寡营养条件下略优于营养条件，并且一般而言，Penicillium chrysogenum、Periconia byssoides 和 Parengyodontium album 显示出最高的平均定植率。此外，大多数测试的物种在矿物分解、位移和可能的溶解方面产生了明显的变化。获得的结果还允许检测某些物种菌丝中或附近的方解石矿化，这表明经过一年的真菌成岩作用和二次生物矿化已经发生。这项研究有助于进一步阐明真菌增殖对白云质石灰岩的有害影响。Periconia byssoides 和 Parengyodontium album 显示出最高的平均定植率。此外，大多数测试的物种在矿物分解、位移和可能的溶解方面产生了明显的变化。获得的结果还允许检测某些物种菌丝中或附近的方解石矿化，这表明经过一年的真菌成岩作用和二次生物矿化已经发生。这项研究有助于进一步阐明真菌增殖对白云质石灰岩的有害影响。Periconia byssoides 和 Parengyodontium album 显示出最高的平均定植率。此外，大多数测试的物种在矿物分解、位移和可能的溶解方面产生了明显的变化。获得的结果还允许检测某些物种菌丝中或附近的方解石矿化，这表明经过一年的真菌成岩作用和二次生物矿化已经发生。这项研究有助于进一步阐明真菌增殖对白云质石灰岩的有害影响。获得的结果还允许检测某些物种菌丝中或附近的方解石矿化，这表明经过一年的真菌成岩作用和二次生物矿化已经发生。这项研究有助于进一步阐明真菌增殖对白云质石灰岩的有害影响。获得的结果还允许检测某些物种菌丝中或附近的方解石矿化，这表明经过一年的真菌成岩作用和二次生物矿化已经发生。这项研究有助于进一步阐明真菌增殖对白云质石灰岩的有害影响。