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PD-1 Expression on CD8+CD28- T cells within inflammatory synovium is associated with Relapse: A cohort of Rheumatoid Arthritis
Immunology Letters ( IF 4.4 ) Pub Date : 2020-10-15 , DOI: 10.1016/j.imlet.2020.10.005
Faezeh Ajam 1 , Mehrdad Aghaei 2 , Saeed Mohammadi 3 , Hadiseh Samiei 4 , Nasser Behnampour 5 , Ali Memarian 6
Affiliation  

Defect in T lymphocyte homeostasis could implicate initiation and development of rheumatoid arthritis (RA). Since PD-1 plays a key role in the regulation of T lymphocytes, its expression pattern in various CD8+ T cell subsets could be so effective in RA pathogenesis. Here, we investigated the expression of PD-1 and CXCR3 on CD8+CD28- T cells in association with the IFN-γ levels in patients with RA. A total of 42 RA patients, including 10 newly-diagnosed (ND) and 32 relapsed (RL) cases and also 20 healthy donors were enrolled. Phenotypic characterization of CD8+ T cells derived from peripheral blood (PB) and synovial fluid (SF) was performed by flow cytometry. The plasma and SF IFN-γ levels were also assessed by ELISA. The frequency of CD8+CD28- T cells showed no significant differences between patients and controls while its higher levels were observed in PB, versus SF of RL patients. Relapsed patients also showed higher CXCR3 and especially PD-1 expression on their CD8+CD28- T cells. The IFN-γ concentration was elevated in SF of ND patients while its plasma level was significantly lower in RL subgroup than controls. Although PD-1 could induce immune suppression in effector T cells, it is upregulated during inflammation and its overexpression on CD8+CD28- T cells within inflammatory synovium is associated with severity of disease in our cohort of RA patients.



中文翻译:

炎症性滑膜内 CD8+CD28-T 细胞的 PD-1 表达与复发有关:一组类风湿性关节炎

T 淋巴细胞稳态缺陷可能与类风湿性关节炎 (RA) 的发生和发展有关。由于 PD-1 在 T 淋巴细胞的调节中起关键作用,其在各种 CD8+ T 细胞亚群中的表达模式在 RA 发病机制中可能如此有效。在这里,我们研究了 PD-1 和 CXCR3 在 CD8+CD28-T 细胞上的表达与 RA 患者中 IFN-γ 水平的相关性。共纳入 42 例 RA 患者,包括 10 例新诊断 (ND) 和 32 例复发 (RL) 病例以及 20 名健康供体。来自外周血 (PB) 和滑液 (SF) 的 CD8+ T 细胞的表型特征通过流式细胞术进行。还通过ELISA评估了血浆和SF IFN-γ水平。CD8+CD28-T 细胞的频率在患者和对照组之间没有显着差异,而在 PB 中观察到其水平高于 RL 患者的 SF。复发患者的 CD8+CD28-T 细胞也显示出更高的 CXCR3,尤其是 PD-1 表达。ND 患者 SF 中 IFN-γ 浓度升高,而 RL 亚组的血浆水平显着低于对照组。尽管 PD-1 可以诱导效应 T 细胞的免疫抑制,但它在炎症过程中被上调,并且它在炎症滑膜内的 CD8+CD28-T 细胞上的过度表达与我们的 RA 患者队列中的疾病严重程度有关。ND 患者 SF 中 IFN-γ 浓度升高,而 RL 亚组的血浆水平显着低于对照组。尽管 PD-1 可以诱导效应 T 细胞的免疫抑制,但它在炎症过程中被上调,并且它在炎症滑膜内的 CD8+CD28-T 细胞上的过度表达与我们的 RA 患者队列中的疾病严重程度有关。ND 患者 SF 中 IFN-γ 浓度升高,而 RL 亚组的血浆水平显着低于对照组。尽管 PD-1 可以诱导效应 T 细胞的免疫抑制,但它在炎症过程中被上调,并且它在炎症滑膜内的 CD8+CD28-T 细胞上的过度表达与我们的 RA 患者队列中的疾病严重程度有关。

更新日期:2020-10-30
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