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Probe A shown in the GeneXpert MTB/RIF assay during the detection of Mycobacterium intracellular infections
Diagnostic Microbiology and Infectious Disease ( IF 2.9 ) Pub Date : 2020-10-15 , DOI: 10.1016/j.diagmicrobio.2020.115243
Yuanyuan Tang 1 , Jing Yu 2 , Guoru Yang 3 , Lanhua Li 4 , Juan Cheng 3 , Haizhi Wang 3 , Guangfu Liu 3 , Zhaobao Pan 3 , Xiaoli Yu 4 , Wanwei Li 4
Affiliation  

Mycobacterium tuberculosis (MTB) is commonly diagnosed via the GeneXpert MTB/RIF assay. The cycle threshold (Ct) value of probe A from this assay produced a fluorescence signal upon Mycobacterium intracellulare detection. No other nontuberculous mycobacteria (NTM) exhibited positive probe signals. Using a confirmed mycobacterial culture as a standard, probe A of the assay exhibited 84% sensitivity (95% confidence interval [CI]: 71%–97%) and 50% specificity (95% CI: 37%–63%) for clinical samples. For M. intracellulare strains, probe A exhibited 90% sensitivity (95% CI: 80%–100%) and 50% specificity (95% CI: 37%–63%). The identity of the amino acid sequence and 81-bp core region of rpoB from MTB and NTM suggested that the highly conserved property might be associated with a mismatch between the probes and the chromosomal DNA target. Probe A yielded a positive signal upon M. intracellulare detection; thus, probe A may help diagnose M. intracellular infections.



中文翻译:

在检测分枝杆菌细胞内感染过程中,GeneXpert MTB / RIF分析中显示的探针A 

结核分枝杆菌(MTB)通常通过GeneXpert MTB / RIF分析进行诊断。来自该测定的探针A的循环阈值(Ct)值在细胞内分枝杆菌检测时产生荧光信号。没有其他非结核分枝杆菌(NTM)表现出阳性探针信号。以确诊的分枝杆菌培养物为标准,该方法的探针A对临床表现出84%的敏感性(95%置信区间[CI]:71%–97%)和50%的特异性(95%CI:37%–63%)样品。对于胞内分枝杆菌菌株,探针A表现出90%的敏感性(95%CI:80%–100%)和50%的特异性(95%CI:37%–63%)。rpoB的氨基酸序列和81-bp核心区的同一性来自MTB和NTM的研究表明,高度保守的特性可能与探针和染色体DNA靶标之间的错配有关。探针A在细胞内分枝杆菌检测后产生阳性信号。因此,探针A可能有助于诊断 M.细胞内感染

更新日期:2020-10-30
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