During development, highly dynamic reconstruction of microtubules is involved in many cellular processes, including cell division, migration, morphological changes, and material transportation within cells. Microtubule severing proteins (MSPs), with the function of cutting microtubules into short parts, are important regulators in the reconstruction of microtubule arrays. Fidgetin (fign) and its family members fidgetin like 1 (fignl1) and fignl2 are MSPs, and knowledge on the expression patterns of fign family members will benefit our understanding of their primary roles in one specific stage during development. In this study, we compared the evolutionary relationships of fign family members and found that fignl2 is closer to fign than fignl1. We utilized the zebrafish model and in situ hybridization (ISH) to parallelly identify the expression features of fign family members. Our findings revealed that before 12 h post fertilization (hpf), the expression patterns of fign and fignl1 and fignl2 genes were similar, but differences arose thereafter. Fignl2 transcripts were present in more tissues and organs of zebrafish after 12 hpf and potentially exhibited more ubiquitous functions. This study is the first to assess systematic comparable data on the expression patterns of fign family members during development

在开发过程中，微管的高动态重建涉及许多细胞过程，包括细胞分裂，迁移，形态变化和细胞内物质运输。具有将微管切成短段的功能的微管切断蛋白（MSP）是微管阵列重建中的重要调节剂。Fidgetin（fign）及其家族成员fidgetin像1（fignl1）和fignl2是MSP，关于fign家族成员表达模式的知识将有助于我们了解它们在发育中的特定阶段的主要作用。在这项研究中，我们比较了fign家族成员的进化关系，发现fignl2比fignl1更接近于fign。我们利用斑马鱼模型和原位杂交（ISH）来平行鉴定Fign家族成员的表达特征。我们的研究结果表明，前12小时后受精（HPF），的表达模式fign和fignl1和fignl2基因是相似的，但此后的差异产生。12 hpf后，Fignl2转录本存在于更多的斑马鱼组织和器官中，并可能表现出更普遍的功能。这项研究是第一个评估发育期无花果家族成员表达方式的系统可比数据