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Detection of MOG-IgG by cell-based assay: moving from discovery to clinical practice
Neurological Sciences ( IF 3.3 ) Pub Date : 2020-10-15 , DOI: 10.1007/s10072-020-04828-1
Amanda Marchionatti , Mark Woodhall , Patrick Joseph Waters , Douglas Kazutoshi Sato

Myelin oligodendrocyte glycoprotein (MOG) is a unique CNS-specific mammalian protein that is expressed on the surface of compact myelin and oligodendrocyte cell bodies. MOG is an accessible target for autoantibodies, associated with immune-mediated demyelination in the central nervous system. The identification of MOG reactive immunoglobulin G antibodies (MOG-IgG) helps to distinguish a subgroup of patients from multiple sclerosis and other CNS disorders, reducing the risk of clinical misdiagnosis. The development of the cell-based assays (CBA) improved the detection of clinically meaningful MOG-IgG binding to conformational MOG expressed in the cell membrane surface. In this review, we describe factors that impact on the results of CBA, such as MOG conformation, protein glycosylation, addition of fluorescent tags, serum dilution, secondary antibodies, and data interpretation.



中文翻译:

通过基于细胞的检测法检测MOG-IgG:从发现到临床实践

髓磷脂少突胶质细胞糖蛋白(MOG)是一种独特的CNS特异性哺乳动物蛋白,在致密的髓鞘和少突胶质细胞体表面表达。MOG是自身抗体的可及靶标,与中枢神经系统中免疫介导的脱髓鞘有关。MOG反应性免疫球蛋白G抗体(MOG-IgG)的鉴定有助于从多发性硬化症和其他中枢神经系统疾病中区分出患者亚组,从而降低了临床误诊的风险。基于细胞的测定法(CBA)的发展改善了对临床有意义的MOG-IgG与细胞膜表面表达的构象型MOG结合的检测。在这篇综述中,我们描述了影响CBA结果的因素,例如MOG构象,蛋白质糖基化,添加荧光标签,血清稀释,

更新日期:2020-10-15
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