Humans can be infected by pathogenic Leptospira through contact with infected animals or contaminated environments. Recreational exposure has been associated with human leptospirosis; however, there is a lack of information on the distribution of Leptospira spp. in recreational areas. Thus, we conducted this study to detect and describe the distributions of Leptospira spp. and to determine the prevalence of pathogenic leptospires at recreational areas in Peninsular Malaysia. Soil and water samples were randomly collected from 33 recreational areas in Peninsular Malaysia from December 2018 to April 2019. Each culture was examined under dark-field microscopy prior to genus confirmation by polymerase chain reaction using primers for the 16S rRNA gene. A total of 390 water and soil samples were collected, and 131 cultures were positive for Leptospira under dark-field microscope examination. Leptospira was identified in most of the recreational areas sampled, and at least one pathogenic Leptospira species was isolated from 17 recreational areas. The prevalence of saprophytic, intermediate, and pathogenic Leptospira spp. was 19.7%, 5.6%, and 8.2%, respectively. The dominant pathogenic species found in the samples was Leptospira kmetyi. This study provides important data on the distribution and prevalence of Leptospira spp. from soil and water, as well as the dominant pathogenic species, at recreational areas in Peninsular Malaysia. Therefore, preventive measures should be taken to ensure the safety of visitors.

接触感染的动物或受污染的环境可能会使人感染致病性钩端螺旋体。娱乐性暴露已与人类钩端螺旋体病相关。然而，关于钩端螺旋体属的分布缺乏信息。在娱乐场所。因此，我们进行了这项研究，以检测和描述钩端螺旋体的分布。并确定致病性钩端螺旋体在马来西亚半岛娱乐区的患病率。从2018年12月至2019年4月，从马来西亚半岛的33个娱乐场所随机收集土壤和水样。每种培养物均在暗视野显微镜下进行检查，然后使用16S引物通过聚合酶链反应确认属rRNA基因。总共收集了390个水和土壤样品，在暗视场显微镜检查下131种培养物钩端螺旋体呈阳性。采样的大部分娱乐场所均鉴定出钩端螺旋体，并从17个娱乐场所中分离出至少一种致病性钩端螺旋体。腐生，中间和致病性钩端螺旋体的患病率。分别为19.7％，5.6％和8.2％。样本中发现的主要致病物种是钩端螺旋体。这项研究提供了有关钩端螺旋体分布和流行的重要数据spp。来自马来西亚半岛休闲区的土壤和水以及主要致病物种。因此，应采取预防措施以确保访客的安全。