Perfluorooctane sulfonate (PFOS) is a legacy contaminant that has been detected globally within the environment and throughout numerous species, including humans. Despite an international ban on its use, this unique contaminant continues to persist in organisms and their surroundings due to PFOS’s inability to breakdown into nontoxic forms resulting in bioaccumulation. In this study, we analyzed the effects of a technical mixture of PFOS (linear and branched isomers) in the adult Eastern oyster, Crassostrea virginica, at 2 days and 7 days exposure. Biomarker analysis (lysosomal destabilization, lipid peroxidation, and glutathione assays) in oyster tissue along with chemical analysis (liquid chromatography tandem mass spectrometry) of PFOS in oyster tissue and water samples revealed the oysters’ ability to overcome exposures without significant damage to lipid membranes or the glutathione phase II enzyme system; however, significant cellular lysosomal damage was observed. The oysters were able to eliminate up to 96% of PFOS at 0.3 mg/L and 3 mg/L exposures when allowed to depurate for 2 days in clean seawater. Chemical analysis showed the linear isomer to be the prevailing fraction of the residual PFOS contained in oyster tissue. Results provide insight into possible detrimental cellular effects of PFOS exposure in addition to offering insight into contaminant persistence in oyster tissue.

全氟辛烷磺酸盐（PFOS）是一种遗留污染物，已在环境中以及包括人类在内的众多物种中被全球检测到。尽管国际上禁止使用它，但由于全氟辛烷磺酸无法分解成无毒形式导致生物蓄积，这种独特的污染物仍继续存在于生物及其周围环境中。在这项研究中，我们分析了全氟辛烷磺酸（线性和分支异构体）技术混合物对成年东部牡蛎Crassostrea virginica的影响，在暴露2天和7天时。牡蛎组织中的生物标志物分析（溶酶体去稳定，脂质过氧化和谷胱甘肽测定）以及牡蛎组织和水样中PFOS的化学分析（液相色谱串联质谱）表明，牡蛎克服暴露的能力不会对脂质膜或脂质造成重大损害谷胱甘肽II期酶系统；然而，观察到明显的细胞溶酶体损伤。当牡蛎在干净的海水中净化2天时，它们能够以0.3 mg / L和3 mg / L的暴露量消除多达96％的PFOS。化学分析表明，线性异构体是牡蛎组织中所含残留全氟辛烷磺酸的主要成分。