The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is the major insect pest of fruit production worldwide. Chemical control of this pest has induced the selection of resistant medflies populations and negative environmental impact. In addition, the use of pesticides has become an obstacle to the fresh agricultural products exported to many countries that restrict pesticides residues. The aim of this study was to propose alternatives based on the use of microbiological products for the control of C. capitata. The pathogenicity of the Turkish isolate Beauveria bassiana BMAUM M6-4 was evaluated against immature stages and adult of C. capitata under laboratory conditions at a concentration of 107 conidia/ml via different routes of exposure. Conidial treatment was applied by spraying soil and filter paper against 3rd instar larvae and pupae. In contrast, the treatment was administered to adult males and females by contact and ingestion. The percentage of mortality was recorded on the 3rd, 7th, 12th and 15th day after inoculation. Obtained results showed that B. bassiana was very effective against the adult’s fly, where males were more susceptible than females in both treatments (contact and ingestion). This isolate was tested against 3rd instar larvae. It achieved an infection rate of 33.33 and 43.5% of the larvae treated on filter paper and in the soil, respectively. Application of conidial preparations to soil reduced insect emergence and CAN represent a promising strategy for fruit fly integrated management (57.33 and 56.66% emergence from the treatments in soil and filter paper). Entomopathogenic fungi are promising as a biocontrol agent that can be used under different modes of ground application against larvae, prepupae and pupae, and/or as a bait spraying or contact against adults.

中文翻译：

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实验室条件下本土球孢白僵菌（Balveria bassiana（Balsamo））对Capetata capedata Wiedemann（Diptera：Tephritidae）的致病性

地中海果蝇（Ceratitis capitata（Wiedemann）（Diptera：Tephritidae））是全世界水果生产的主要害虫。对这种有害生物的化学防治已导致对抗性中等居群的选择和对环境的不利影响。此外，农药的使用已经成为向许多限制农药残留的国家出口新鲜农产品的障碍。这项研究的目的是基于微生物产品的控制提出建议替代方案。通过不同的暴露途径，在实验室条件下以107分生孢子/ ml的浓度评估土耳其分离球孢白僵菌BMAUM M6-4对未成熟阶段和成年衣原体成虫的致病性。通过对第三龄幼虫和p喷洒土壤和滤纸进行分生孢子处理。相反，该治疗是通过接触和摄入对成年男性和女性进行的。在接种后第3、7、12和15天记录死亡率。获得的结果表明，球孢白僵菌对成年苍蝇非常有效，在两种治疗（接触和摄入）下，雄性比雌性更易感染雌性。该分离物针对三龄幼虫进行了测试。在滤纸上和在土壤中处理的幼虫的感染率分别为33.33和43.5％。分生孢子制剂在土壤中减少虫害的发生和CAN的应用代表了果蝇综合治理的有前途的策略（从土壤和滤纸处理中出现的57.33和56.66％）。