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Comparative carcinogenicity study of a thick, straight-type and a thin, tangled-type multi-walled carbon nanotube administered by intra-tracheal instillation in the rat
Particle and Fibre Toxicology ( IF 10 ) Pub Date : 2020-10-15 , DOI: 10.1186/s12989-020-00382-y
Dina Mourad Saleh 1, 2, 3 , William T Alexander 1 , Takamasa Numano 1 , Omnia Hosny Mohamed Ahmed 1, 2, 4 , Sivagami Gunasekaran 1, 2 , David B Alexander 1 , Mohamed Abdelgied 1, 2, 5 , Ahmed M El-Gazzar 1, 2, 6 , Hiroshi Takase 7 , Jiegou Xu 1, 8 , Aya Naiki-Ito 2 , Satoru Takahashi 2 , Akihiko Hirose 9 , Makoto Ohnishi 10 , Jun Kanno 10 , Hiroyuki Tsuda 1
Affiliation  

Multi-walled carbon nanotubes can be divided into two general subtypes: tangled and straight. MWCNT-N (60 nm in diameter) and MWCNT-7 (80–90 nm in diameter) are straight-type MWCNTs, and similarly to asbestos, both are carcinogenic to the lung and pleura when administered to rats via the airway. Injection of straight-type MWCNTs into the peritoneal cavity also induces the development of mesothelioma, however, injection of tangled-type MWCNTs into the peritoneal cavity does not induce carcinogenesis. To investigate these effects in the lung we conducted a 2-year comparative study of the potential carcinogenicities of a straight-type MWCNT, MWCNT-A (approximately 150 nm in diameter), and a tangled-type MWCNT, MWCNT-B (7.4 nm in diameter) after administration into the rat lung. Crocidolite asbestos was used as the reference material, and rats administered vehicle were used as the controls. Test materials were administered by intra-Tracheal Intra-Pulmonary Spraying (TIPS) once a week over a 7 week period (8 administrations from day 1 to day 50), followed by a 2-year observation period without further treatment. Rats were administered total doses of 0.5 or 1.0 mg MWCNT-A and MWCNT-B or 1.0 mg asbestos. There was no difference in survival between any of the groups. The rats administered MWCNT-A or asbestos did not have a significant increase in bronchiolo-alveolar hyperplasia or tumors in the lung. However, the rats administered MWCNT-B did have significantly elevated incidences of bronchiolo-alveolar hyperplasia and tumors in the lung: the incidence of bronchiolo-alveolar hyperplasia was 0/20, 6/20, and 9/20 in the vehicle, 0.5 mg MWCNT-B, and 1.0 mg MWCNT-B groups, respectively, and the incidence of adenoma and adenocarcinoma combined was 1/19, 5/20, and 7/20 in the vehicle, 0.5 mg MWCNT-B, and 1.0 mg MWCNT-B groups, respectively. Malignant pleural mesothelioma was not induced in any of the groups. The results of this initial study indicate that tangled-type MWCNT-B is carcinogenic to the rat lung when administered via the airway, and that straight-type MWCNT-A did not have higher carcinogenic potential in the rat lung than tangled-type MWCNT-B.

中文翻译:

气管内滴注法对大鼠厚,直型和薄型,缠结型多壁碳纳米管致癌性的比较研究

多壁碳纳米管可分为两种一般的子类型:缠结和笔直。MWCNT-N(直径为60 nm)和MWCNT-7(直径为80-90 nm)是直型MWCNT,与石棉相似,当通过气道对大鼠给药时,它们对肺和胸膜均具有致癌性。将直型MWCNTs注入腹膜腔也会诱发间皮瘤的发展,但是,将纠结型MWCNTs注入腹膜腔不会诱发癌变。为了研究这些对肺部的影响,我们进行了为期两年的对比研究,研究了直型MWCNT MWCNT-A(直径约150 nm)和缠结型MWCNT MWCNT-B(7.4 nm)的潜在致癌性。直径)注入大鼠肺后。使用鳄鱼蓝石棉作为参考材料,用给予媒介物的大鼠作为对照。在7周的时间内每周一次通过气管内肺内喷雾(TIPS)施用测试材料(从第1天到第50天共8次施用),然后进行为期2年的观察期,无需进一步治疗。给大鼠施用0.5或1.0mg MWCNT-A和MWCNT-B或1.0mg石棉的总剂量。两组之间的生存率均无差异。施用MWCNT-A或石棉的大鼠的支气管肺泡增生或肺部肿瘤没有明显增加。但是,给予MWCNT-B的大鼠确实有明显升高的支气管肺泡增生和肺部肿瘤的发生:载体中的支气管肺泡增生的发生率为0 / 20、6 / 20和9 / 20,0.5 mg MWCNT-B和1.0 mg MWCNT-B组,媒介物,0.5 mg MWCNT-B和1.0 mg MWCNT-B组中,腺瘤和腺癌合并的发生率分别为1 / 19、5 / 20和7/20。任何一组均未诱发恶性胸膜间皮瘤。这项初步研究的结果表明,缠结型MWCNT-B在通过气道给药时对大鼠肺具有致癌性,而直型MWCNT-A在大鼠肺中的致癌潜力并不比缠结型MWCNT-A高。 B.
更新日期:2020-10-15
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