Electro-active conducting hydrogels have shown promising applications in promoting soft tissue regeneration. However, achieving good conductive performance while simultaneously imparting macroporous structures to these hydrogels still remains challenging. In this study, we report the development of multifunctional conductive macroporous nanocomposite hydrogels (MNHs) prepared by an air-in-water emulsion template that is stabilized by colloidal hybrids of carbon nanotubes (CNTs) and gelatin methacryloyl. The MNH hydrogels demonstrated tunable pore size, electrical conductivity and mechanical properties with various CNT concentrations in the crosslinking matrices. An in vitro cell assay showed that the MNH hydrogels could promote the spreading and differentiation of NE-4C neural stem cells. Furthermore, sustainable release of antimicrobial peptides (AMPs) from the MNH hydrogel can be achieved and the released AMPs maintained high S. aureus killing activity. An in vivo evaluation of the MNH hydrogel using a murine dorsal skin model further showed that the conductive MNH hydrogel loaded with AMPs demonstrated appealing antimicrobial and wound healing performance in two weeks.

中文翻译：

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由水包空气Pickering乳液产生的导电和抗菌大孔纳米复合水凝胶，用于神经干细胞分化和皮肤伤口愈合

电活性导电水凝胶在促进软组织再生方面显示出有希望的应用。然而，实现良好的导电性能同时向这些水凝胶赋予大孔结构仍然是挑战。在这项研究中，我们报告了由水-空气乳液模板制备的多功能导电大孔纳米复合水凝胶（MNH）的发展，该模板由碳纳米管（CNT）和明胶甲基丙烯酰的胶体杂化物稳定。MNH水凝胶在交联基质中具有各种CNT浓度时，具有可调的孔径，电导率和机械性能。一种在体外细胞分析表明，MNH水凝胶可促进NE-4C神经干细胞的扩散和分化。此外，可以实现从MNH水凝胶可持续释放抗菌肽（AMP），并且释放的AMP保持了高的金黄色葡萄球菌杀伤活性。的体内利用鼠背部皮肤模型的水凝胶MNH的评价进一步表明，装载有抗菌肽导电MNH水凝胶表现出吸引人的抗微生物和缠绕在两个星期内愈合性能。