Objective

Since the systemic drugs have been used to reduce the hyperplasic response in the tunica intima, the periadventitial local drug applications to the vascular wall have gained more popularity. In this study, we investigated the effect of bovine serum albumin-glutaraldehyde and polyethylene glycol polymer on neointimal hyperplasia in rabbit carotid artery anastomosis to explore the effects of these two different agents.

Methods

21 New Zealand male rabbits were randomly divided into three groups. The carotid artery transection and anastomosis was performed onthe control group. The bovine serum albumin-glutaraldehyde and the polyethylene glycol polymer were applied locally on the other two groups seperatley after transection and anastomosis of the carotid arteries. At the end of 28-day follow-up, the histological and the immunohistochemical results related to neointimal hyperplasia were compared.

Results

The glue residues were detected in the BSA-glutaraldehyde group, but in the PEG polymer group there was no glue residue. The intima thickness and the intima/media thickness ratio in the control group was significantly higher (p<0.05) than the other groups. These values did not differ significantly between the BSA-glutaraldehyde group and the PEG polymer group (p>0.05). The lumen diameter and the area in the control group were significantly higher (p < 0.05) than the BSA-glutaraldehyde group. These values between the control group and the PEG polymer group did not differ significantly (p>0.05). aSMA-positive staining score in the Control group was found to be significantly lower (p < 0.05) than the BSA-glutaraldehyde and PEG polymer group and the VEGF-positive staining score in the control group was found to be significantly higher (p < 0.05) than the BSA-glutaraldehyde and the PEG polymer group.

Conclusions

Although the both agents have positive results on neointimal hyperplasia, it would be favorable to use polyethylene glycol polymer, since it does not seem to affect the lumen area and the lumen diameter of the vessel.

中文翻译：

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牛血清白蛋白-戊二醛和聚乙二醇聚合物对兔颈动脉吻合术中新生内膜增生的影响

客观的

由于全身性药物已被用于减少内膜中的增生反应，外膜周围局部药物应用于血管壁已越来越受欢迎。在这项研究中，我们研究了牛血清白蛋白-戊二醛和聚乙二醇聚合物对兔颈动脉吻合术中新生内膜增生的影响，以探索这两种不同药物的作用。

方法

21只新西兰雄性兔随机分为三组。对照组行颈总动脉横断吻合术。其他两组分别在颈动脉横断吻合后局部应用牛血清白蛋白-戊二醛和聚乙二醇聚合物。28天随访结束时，比较与新生内膜增生相关的组织学和免疫组化结果。

结果

在BSA-戊二醛组中检测到胶残留，但在PEG聚合物组中没有胶残留。对照组的内膜厚度和内膜/中膜厚度比明显高于其他组（p<0.05）。这些值在 BSA-戊二醛组和 PEG 聚合物组之间没有显着差异 (p>0.05)。对照组的管腔直径和面积显着高于（p < 0.05）BSA-戊二醛组。对照组和PEG聚合物组之间的这些值没有显着差异（p>0.05）。对照组的 aSMA 阳性染色评分显着降低（p < 0.

结论

虽然这两种药物对新生内膜增生都有积极的效果，但使用聚乙二醇聚合物是有利的，因为它似乎不会影响血管的管腔面积和管腔直径。