Osteochondral tissue repair represents a common clinical need, with multiple approaches in tissue engineering and regenerative medicine being investigated for the repair of defects of articular cartilage and subchondral bone. A full thickness rabbit femoral condyle defect is a clinically relevant model of an articulating and load bearing joint surface for the investigation of osteochondral tissue repair by various cell-, biomolecule-, and biomaterial-based implants. In this protocol, we describe the methodology and 1.5- to 2-h surgical procedure for the generation of a reproducible, full thickness defect for construct implantation in the rabbit medial femoral condyle. Furthermore, we describe a step-by-step procedure for osteochondral tissue collection and the assessment of tissue formation using standardized histological, radiological, mechanical, and biochemical analytical techniques. This protocol illustrates the critical steps for reproducibility and minimally invasive surgery as well as applications to evaluate the efficacy of cartilage and bone tissue engineering implants, with emphasis on the usage of histological and radiological measures of tissue growth.

中文翻译：

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用于评估骨软骨组织再生的兔股骨髁缺损模型

骨软骨组织修复代表了一种常见的临床需求，正在研究组织工程和再生医学中的多种方法来修复关节软骨和软骨下骨的缺陷。全厚度兔股骨髁缺损是关节和承重关节面的临床相关模型，用于研究各种细胞、生物分子和生物材料植入物对骨软骨组织的修复。在该协议中，我们描述了用于在兔股骨内侧髁中构建植入的可重复、全厚度缺陷的生成方法和 1.5 至 2 小时的手术程序。此外，我们描述了使用标准化组织学、放射学、机械学、和生化分析技术。该协议说明了可重复性和微创手术的关键步骤，以及评估软骨和骨组织工程植入物功效的应用，重点是组织生长的组织学和放射学测量的使用。