Mitogen-activated protein kinases (MAPK) play pivotal roles in transducing developmental cues and environmental signals into cellular responses through pathways initiated by MAPK kinase kinases (MAP3K). AtYODA is a MAP3K of Arabidopsis thaliana that controls stomatal development and non-canonical immune responses. Arabidopsis plants overexpressing a constitutively active YODA protein (AtCA-YDA) show broad-spectrum disease resistance and constitutive expression of defensive genes. We tested YDA function in crops immunity by heterologously overexpressing AtCA-YDA in Solanum lycopersicum. We found that these tomato AtCA-YDA plants do not show developmental phenotypes and fitness alterations, except a reduction in stomatal index, as reported in Arabidopsis AtCA-YDA plants. Notably, AtCA-YDA tomato plants show enhanced resistance to the bacterial pathogen Pseudomonas syringae pv. tomato DC3000 and constitutive upregulation of defense-associated genes, corroborating the functionality of YDA in tomato immunity. This function was further supported by generating CRISPR/Cas9-edited tomato mutants impaired in the closest orthologs of AtYDA [Solyc08g081210 (SlYDA1) and Solyc03g025360 (SlYDA2)]. Slyda1 and Slyda2 mutants are highly susceptible to P. syringae pv. tomato DC3000 in comparison to wild-type plants but only Slyda2 shows altered stomatal index. These results indicate that tomato orthologs have specialized functions and support that YDA also regulates immune responses in tomato and may be a trait for breeding disease resistance.

丝裂原激活的蛋白激酶（MAPK）在通过MAPK激酶激酶（MAP3K）引发的途径将发育线索和环境信号转化为细胞反应中起关键作用。AtYODA是的MAP3K拟南芥 控制气孔发育和非典型的免疫反应。 拟南芥 过表达组成型活性YODA蛋白（亚特兰大）显示了广谱抗病性和防御基因的组成型表达。我们通过异源过表达测试了YDA在农作物免疫中的功能亚特兰大 在 番茄。我们发现这些番茄亚特兰大 植物的生长表型和适应性没有改变，除了气孔指数的降低， 拟南芥AtCA-YDA植物。值得注意的是亚特兰大 番茄植株对细菌病原体的抵抗力增强 丁香假单胞菌 pv。 番茄DC3000和防御相关基因的组成型上调，证实了YDA在番茄免疫中的功能。通过产生在最近的直系同源物中受损的CRISPR / Cas9编辑的番茄突变体进一步支持了该功能。雅达 [Solyc08g081210 （SlYDA1）和 Solyc03g025360 （SlYDA2）]。 Slyda1 和 Slyda2 突变体对 丁香假单胞菌 pv。 番茄 DC3000与野生型植物相比，但仅 Slyda2显示气孔指数改变。这些结果表明，番茄直系同源物具有专门的功能，并支持YDA也调节番茄的免疫反应，并且可能是抗病育种的一个特征。