Abscisic acid (ABA) plays a crucial role in various aspects of plant growth and development, including fruit development and ripening, seed dormancy, and involvement in response to various environmental stresses. In almost all higher plants, ABA signal transduction requires three core components; namely, PYR/PYL/RCAR ABA receptors (PYLs), type 2C protein phosphatases (PP2Cs), and class III SNF-1-related protein kinase 2 (SnRK2s). The exploration of these three core components is not comprehensive in soybean. This study identified the GmPYL-PP2C-SnRK2s gene family members by using the JGI Phytozome and NCBI database. The gene family composition, conservation, gene structure, evolutionary relationship, cis-acting elements of promoter regions, and its coding protein domains were analyzed. In the entire genome of the soybean, there are 21 PYLs, 36 PP2Cs, and 21 SnRK2s genes; further, by phylogenetic and conservation analysis, 21 PYLs genes are classified into 3 groups, 36 PP2Cs genes are classified into seven groups, and 21 SnRK2s genes are classified into 3 groups. The conserved motifs and domain analysis showed that all the GmPYLs gene family members contain START-like domains, the GmPP2Cs gene family contains PP2Cc domains, and the GmSnRK2s gene family contains S_TK domains, respectively. Furthermore, based on the high-throughput transcriptome sequencing data, the results showed differences in the expression patterns of GmPYL-PP2C-SnRK2s gene families in different tissue parts of the same variety, and the same tissue part of different varieties. Our study provides a basis for further elucidation of the identification of GmPYL-PP2C-SnRK2s gene family members and analysis of their evolution and expression patterns, which helps to understand the molecular mechanism of soybean response to abiotic stress. In addition, this provides a conceptual basis for future studies of the soybean ABA core signal pathway.

中文翻译：

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大豆PYL-PP2C-SnRK2s基因家族的鉴定，进化与表达分析

脱落酸（ABA）在植物生长发育的各个方面都发挥着至关重要的作用，包括果实发育和成熟，种子休眠以及参与应对各种环境胁迫。在几乎所有高等植物中，ABA信号转导都需要三个核心成分。即PYR / PYL / RCAR ABA受体（PYLs），2C型蛋白磷酸酶（PP2Cs）和III类SNF-1相关蛋白激酶2（SnRK2s）。在大豆中，这三个核心成分的探索尚不全面。本研究通过使用JGI Phytozome和NCBI数据库鉴定了GmPYL-PP2C-SnRK2s基因家族成员。基因家族组成，保守性，基因结构，进化关系，顺式分析了启动子区域的功能元件及其编码蛋白结构域。在大豆的整个基因组中，有21个PYLs，36个PP2Cs和21个SnRK2s基因。此外，通过系统进化和保守性分析，将21个PYLs基因分为3组，将36个PP2Cs基因分为7组，将21个SnRK2s基因分为3组。保守的基序和结构域分析表明，所有GmPYLs基因家族成员均包含START-like域，GmPP2Cs基因家族包含PP2Cc域，而GmSnRK2s基因家族分别包含S_TK结构域。此外，基于高通量转录组测序数据，结果显示，GmPYL-PP2C-SnRK2s基因家族在相同品种的不同组织部分以及不同品种的相同组织部分中表达模式的差异。本研究为进一步阐明GmPYL-PP2C-SnRK2s基因家族成员及其进化和表达方式提供了基础，有助于了解大豆对非生物胁迫的分子机制。另外，这为将来大豆ABA核心信号途径的研究提供了概念基础。