The microbiota of human skin is influenced by host and environmental factors. To determine if chronological age influences the composition of the skin microbiota on the forehead and hands, 73 Korean women were sorted into one of three age groups: (1) 10–29 years (n = 24), (2) 30–49 years (n = 21), and (3) 50–79 years (n = 28). From the 73 women, 146 skin samples (two skin sites per person) were collected. 16S rRNA gene amplicon sequencing was then conducted to analyze the skin microbiota. The overall microbial distribution varied on the forehead but was similar on the hands across the three age groups. In addition, the composition of the skin microbiota differed between the forehead and hands. Commensal microbiota, such as Streptococcus, Staphylococcus, Cutibacterium, and Corynebacterium, which contribute to maintaining skin health via dominant occupation, were affected by increasing age on forehead and hand skin. Alpha diversity indices increased significantly with age on forehead skin. This study indicates that older people may be more susceptible to pathogenic invasions due to an imbalanced skin microbiota resulting from age-related changes. The results of our study may help develop new strategies to rebalance skin microbiota shifted during aging.

中文翻译：

---

韩国女性皮肤微生物群年龄相关变化的调查

人体皮肤的微生物群受宿主和环境因素的影响。为了确定年龄是否会影响额头和手部皮肤微生物的组成，将73名韩国女性分为以下三个年龄组之一：（1）10-29岁（n = 24），（2）30-49岁（n = 21）和（3）50-79年（n= 28）。从73名妇女中收集了146个皮肤样本（每人两个皮肤部位）。然后进行16S rRNA基因扩增子测序以分析皮肤微生物群。整个微生物分布在前额上有所不同，但在三个年龄组中的手上相似。此外，额头和手的皮肤微生物群组成也不同。前额和手部皮肤年龄的增加会影响共生微生物，例如链球菌，葡萄球菌，皮肤杆菌和棒状杆菌，这些细菌通过占优势的职业有助于维持皮肤健康。随着年龄的增长，前额皮肤的阿尔法多样性指数显着增加。这项研究表明，由于年龄相关的变化导致皮肤微生物群失衡，老年人可能更容易受到病原体侵袭。