In clinical practice, administration of low ozone (O₃) dosages is a complementary therapy for many diseases, due to the capability of O₃ to elicit an antioxidant response through the Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2)-dependent pathway. Nrf2 is also involved in the adipogenic differentiation of mesenchymal stem cells, and low O₃ concentrations have been shown to stimulate lipid accumulation in human adipose-derived adult stem cells in vitro. Thus, O₃ treatment is a promising procedure to improve the survival of explanted adipose tissue, whose reabsorption after fat grafting is a major problem in regenerative medicine. In this context, we carried out a pilot study to explore the potential of mild O₃ treatment in preserving explanted murine adipose tissue in vitro. Scanning and transmission electron microscopy, Western blot, real-time polymerase chain reaction and nuclear magnetic resonance spectroscopy were used. Exposure to low O₃ concentrations down in the degradation of the explanted adipose tissue and induced a concomitant increase in the protein abundance of Nrf2 and in the expression of its target gene Hmox1. These findings provide a promising background for further studies aimed at the clinical application of O₃ as an adjuvant treatment to improve fat engraftment.

中文翻译：

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臭氧激活Nrf2途径并改善离体脂肪组织的体外保存

在临床实践中，由于O ₃具有通过核因子类红细胞2相关因子2（Nrf2）依赖性途径引发抗氧化反应的能力，因此低剂量臭氧（O ₃）的给药是许多疾病的补充疗法。Nrf2也参与间充质干细胞的成脂分化，并且低O ₃浓度已显示在体​​外刺激人脂肪来源的成年干细胞中脂质的积累。因此，O ₃处理是提高外植脂肪组织存活率的有前途的方法，脂肪移植后脂肪组织的重吸收是再生医学的主要问题。在这种情况下，我们进行了一项试点研究，以探索轻度O ₃的潜力在体外保存离体鼠脂肪组织的治疗。使用扫描和透射电子显微镜，蛋白质印迹，实时聚合酶链反应和核磁共振波谱。暴露于低O ₃浓度会降低外植脂肪组织的降解，并导致Nrf2的蛋白质丰度及其目标基因Hmox1的表达随之增加。这些发现为进一步研究以O ₃作为改善脂肪植入的辅助治疗方法的临床应用提供了有希望的背景。