Abstract

The oral squamous cell carcinoma (OSCC) is the eighth more common cancer in men. The development of new and more efficient drugs is needed. Plants of the genus Piper are popularly used in the treatment of many diseases. This study evaluated the antitumor effect of extract, fraction and isolated compounds from leaves of P. rivinoides in oral cancer. The isolated compounds (conocarpan, eupomatenoid-5 and eupomatenoid-6) were effective in inducing cell death in OSCC cell lines (SCC4, SCC9 and SCC25) compared to the standard chemotherapeutic agent carboplatin, and this effect was time-dependent. Conocarpan was more selective and stable than eupomatenoid-5 and eupomatenoid-6, resembling the stability of carboplatin. There was a significant presence of pyknotic nuclei and active caspase-3 expression under conocarpan treatment, suggesting cell death through apoptosis. In conclusion, conocarpan was the most effective compound against OSCC cells and might be considered for future cancer studies.

摘要

口腔鳞状细胞癌 (OSCC) 是男性中第八常见的癌症。需要开发新的和更有效的药物。胡椒属植物广泛用于治疗许多疾病。本研究评估了来自P. rivinoides叶子的提取物、馏分和分离化合物的抗肿瘤作用在口腔癌中。与标准化疗药物卡铂相比，分离的化合物（conocarpan、eupomatenoid-5 和 eupomatenoid-6）可有效诱导 OSCC 细胞系（SCC4、SCC9 和 SCC25）中的细胞死亡，并且这种作用具有时间依赖性。Conocarpan 比 eupomatenoid-5 和 eupomatenoid-6 更具选择性和稳定性，类似于卡铂的稳定性。在 conocarpan 处理下存在显着的固缩核和活性 caspase-3 表达，表明细胞通过细胞凋亡而死亡。总之，椰子油是对抗 OSCC 细胞最有效的化合物，可能会被考虑用于未来的癌症研究。