Protein post‐translational modifications (PTMs) are chemical modifications on proteins. PTMs play a key role in many cellular processes by influencing the structure of proteins and dynamically regulating their functions. Therefore, characterizing PTMs at proteome level is critical to provide invaluable insight into the functions of proteins underlying different biological processes. Advances in modern proteomics technologies including sample preparation, chromatography separation as well as mass spectrometry have propelled the PTMs proteome to further depths. During the past decade, to better examine the PTMs with high sensitivity and selectivity, our group have developed a series of MS‐based novel analytical approaches to study the protein PTMs. Herein, we mainly introduce these approaches developed by our group and discuss how to overcome the technical obstacles of studying various protein PTMs with mass spectrometry.

中文翻译：

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使用质谱发现翻译后修饰蛋白质组

蛋白质翻译后修饰（PTM）是蛋白质的化学修饰。PTM通过影响蛋白质的结构并动态调节其功能在许多细胞过程中发挥关键作用。因此，在蛋白质组水平上表征PTM对于提供有关不同生物学过程中蛋白质功能的宝贵见解至关重要。包括样品制备，色谱分离和质谱在内的现代蛋白质组学技术的进步推动了PTMs蛋白质组的发展。在过去的十年中，为了更好地检查具有高灵敏度和选择性的PTM，我们小组开发了一系列基于MS的新颖分析方法来研究蛋白质PTM。在此处，