Auricularia auricula polysaccharide (AAP) was isolated by hot‐water extraction and purified to evaluate its structural and immunomodulatory effects on RAW264.7 macrophages. The results show that three kinds of Auricularia auricula polysaccharides (c‐AAP) were obtained and named as AAP‐I, AAP‐II, and AAP‐III Their further purification found that AAP‐I and AAP‐II were glycoproteins, and only AAP‐III was a pure polysaccharide, which we named AAP for further experiments. Structural characteristics revealed that AAP was a homogeneous galactan comprising mannose, rhamnose, gluconic acid, glucose, galactose, arabinose, and fucose in a molar ratio of 5.02:0.9:0.12:4.48:0.37:1.0:0.36, and the average molecular weight is approximately 23.51 kDa. Methylation analysis revealed that AAP mainly consisted of 1,4‐linked‐Glcp, 1,4,6‐linked‐Glcp, terminal Glcp, 1,4‐linked‐Manp, 1,2,6‐linked‐Manp, and terminal Arap. Furthermore, the in vitro immunomodulatory activities of AAP were evaluated by cell proliferation, NO production, and phagocytic ability using RAW264.7 macrophage cells. The results show that AAP not only promoted the activation of macrophages but also provided a scientific basis for the further use of AAP.

中文翻译：

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黑木耳多糖对RAW264.7巨噬细胞的分离，纯化，表征及免疫调节作用

通过热水提取分离出极耳木耳多糖（AAP），并进行纯化以评估其对RAW264.7巨噬细胞的结构和免疫调节作用。结果表明，三种木耳获得了多糖（c-AAP）并命名为AAP-I，AAP-II和AAP-III。他们进一步纯化发现，AAP-I和AAP-II是糖蛋白，只有AAP-III是纯多糖，我们命名为AAP进行进一步的实验。结构特征表明，AAP是均一的半乳聚糖，包含甘露糖，鼠李糖，葡萄糖酸，葡萄糖，半乳糖，阿拉伯糖和岩藻糖，摩尔比为5.02：0.9：0.12：4.48：0.37：1.0：0.36，平均分子量为约23.51 kDa。甲基化分析表明AAP主要由1,4-联-Glc p，1,4,6-联-Glc p，末端Glc p，1,4-联-Man p，1,2,6-联-Man组成p和终端Ara p。此外，使用RAW264.7巨噬细胞通过细胞增殖，NO产生和吞噬能力评估了AAP的体外免疫调节活性。结果表明，AAP不仅促进了巨噬细胞的活化，而且为进一步使用AAP提供了科学依据。