The amplification of nucleic acids is a fundamental tool utilized in various scientific disciplines, including Molecular Biology, Immunology, Microbiology, and Genetics. However, due to the time and technology required for traditional polymerase chain reaction and its derivatives, it is not always possible to include such methodologies in undergraduate laboratory curricula. Loop-mediated isothermal amplification (LAMP), a technology that has become increasingly utilized in a variety of laboratory and field settings during the past two decades, is an alternate method of nucleic acid amplification that is rapid, sensitive, and performed under isothermal conditions. We describe an adaptable, inquiry-driven laboratory module that is focused on the detection of Escherichia coli DNA via LAMP amplification. The main objectives of the module are to introduce students to the principles and protocols of LAMP, to help students develop the ability to apply the scientific method to scientific questions, to guide students as they develop the ability to identify the most appropriate methodology to use in the investigation of scientific questions, and to train students to critically evaluate scientific data, theories, and principles and to articulate their evaluations in both written and oral formats.

中文翻译：

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利用环介导等温扩增检测大肠杆菌的存在：一个探究驱动的本科实验室模块

核酸的扩增是用于各种科学学科的基本工具，包括分子生物学、免疫学、微生物学和遗传学。然而，由于传统聚合酶链反应及其衍生物所需的时间和技术，在本科实验室课程中并不总是可能包含此类方法。环介导等温扩增 (LAMP) 是一种在过去二十年中越来越多地用于各种实验室和现场环境的技术，是一种快速、灵敏且在等温条件下进行的核酸扩增的替代方法。我们描述了一个适应性强、以查询为驱动的实验室模块，该模块专注于通过 LAMP 扩增检测大肠杆菌 DNA。