Whole-cell bacterial biosensors can be applied for the screening of antibacterial properties of extracts. We constructed a biosensor panel consisting of four different bacterial biosensor strains: Escherichia, Staphylococcus, Acinetobacter and Pseudomonas for expanded screening potential. The functionality of the panel was first evaluated with known antibacterial compounds: ethanol, naphthoquinones (juglone, lawsone, plumbagin) and a flavonoid (quercetin). Natural extracts comprise a vast source of potential new antibacterials for diverse functional purposes. To demonstrate the utilization of the panel for screening of a demanding sample material, round-leaved sundew (Drosera rotundifolia) extracts were used as an example. Differences between field- and laboratory originating sundew extracts could be detected. This demonstrates the efficiency of the developed biosensor panel in the rapid screening of the antibacterial properties of plant extracts.

全细胞细菌生物传感器可用于筛选提取物的抗菌特性。我们构建了一个由四种不同细菌生物传感器菌株组成的生物传感器面板：埃希氏菌、葡萄球菌、不动杆菌和假单胞菌，以扩大筛选潜力。该面板的功能首先用已知的抗菌化合物进行评估：乙醇、萘醌（胡桃酮、罗松、白花素）和类黄酮（槲皮素）。天然提取物包含大量潜在的新型抗菌剂，可用于多种功能用途。为了展示该面板用于筛选要求苛刻的样品材料圆叶茅膏菜（Drosera rotundifolia) 提取物被用作示例。可以检测到田间和实验室来源的毛茅提取物之间的差异。这证明了开发的生物传感器面板在快速筛选植物提取物的抗菌特性方面的效率。